182101

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2437286..2437923 Replicon chromosome
Accession NZ_CP065137
Organism Bacillus sp. A1(2020)

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IRJ20_RS12265 Protein ID WP_003156187.1
Coordinates 2437573..2437923 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag IRJ20_RS12260 Protein ID WP_003156188.1
Coordinates 2437286..2437567 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRJ20_RS12240 2433651..2434250 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
IRJ20_RS12245 2434343..2434708 + 366 WP_007410229.1 holo-ACP synthase -
IRJ20_RS12250 2434873..2435880 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
IRJ20_RS12255 2435997..2437166 + 1170 WP_039252648.1 alanine racemase -
IRJ20_RS12260 2437286..2437567 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IRJ20_RS12265 2437573..2437923 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IRJ20_RS12270 2438041..2438862 + 822 WP_003156182.1 STAS domain-containing protein -
IRJ20_RS12275 2438867..2439232 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
IRJ20_RS12280 2439235..2439636 + 402 WP_003156178.1 anti-sigma regulatory factor -
IRJ20_RS12285 2439648..2440655 + 1008 WP_039252645.1 PP2C family protein-serine/threonine phosphatase -
IRJ20_RS12290 2440719..2441048 + 330 WP_031378636.1 anti-sigma factor antagonist -
IRJ20_RS12295 2441045..2441527 + 483 WP_007609591.1 anti-sigma B factor RsbW -
IRJ20_RS12300 2441493..2442281 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
IRJ20_RS12305 2442281..2442883 + 603 WP_039252641.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T182101 WP_003156187.1 NZ_CP065137:2437573-2437923 [Bacillus sp. A1(2020)]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T182101 NZ_CP086326:c204652-204392 [Lacticaseibacillus rhamnosus]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT182101 WP_003156188.1 NZ_CP065137:2437286-2437567 [Bacillus sp. A1(2020)]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT182101 NZ_CP086326:c204914-204645 [Lacticaseibacillus rhamnosus]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References