Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4767901..4768398 Replicon chromosome
Accession NZ_CP065136
Organism Escherichia coli strain EE72d

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I2T83_RS22965 Protein ID WP_001173343.1
Coordinates 4767901..4768074 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I2T83_RS22970 Protein ID WP_000398619.1
Coordinates 4768102..4768398 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2T83_RS22955 4764571..4766028 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
I2T83_RS22960 4766265..4767782 + 1518 WP_000003806.1 lysine--tRNA ligase -
I2T83_RS22965 4767901..4768074 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I2T83_RS22970 4768102..4768398 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I2T83_RS22975 4768627..4768899 - 273 WP_000405647.1 N-acetyltransferase -
I2T83_RS22980 4768911..4769141 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
I2T83_RS22985 4769322..4770953 + 1632 WP_001216477.1 sensor histidine kinase -
I2T83_RS22990 4770950..4771669 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T182099 WP_001173343.1 NZ_CP065136:c4768074-4767901 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T182099 NZ_CP086305:2412578-2412844 [Burkholderia ambifaria]
ATGAGCGTACGCCGCGTGCTCTTCACTTCCGACGCGCGGGACGACTACGTTTATTGGCAAGGGCAAGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAGCCCAACGGACGCCGTTCGAAGGCATCGGCAAACCCGAACCACTGAAGGCCA
ATCTGTCCGGTTTCTGGTCACGTCGCATCGACGATACGAACCGTCTCGTCTACGAAGCGGACGATCTTCAGATCAGCATC
GTGTCCTGCCGCTATCACTACGCGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT182099 WP_000398619.1 NZ_CP065136:c4768398-4768102 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT182099 NZ_CP086305:2412321-2412581 [Burkholderia ambifaria]
ATGCGCACGATTCACTTTTCGGATGCCCGCGGCAATCTGAAGACGGTCATCGACCAGGTCGTCGACGACGCGGACGTGAC
GCTCATCACACGTCGCGACGCGCCGAACGCCGTCATCATGTCGCAGGAATACTACGACAGCCTGATGGAGACGGTTCACC
TGCTCCGCTCGCCGGCCAACGTCGCCCACCTCGAACGCTCGATTGCGCAATTGCGCAAGGGCAAGGCGAAAGAGCACAAG
CTGGCGGAGGACGATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References