Detailed information of TA system TA182098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4670347..4670942
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: I2T83_RS22460
• Protein ID: WP_000239581.1
• Coordinates: 4670347..4670697 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: I2T83_RS22465
• Protein ID: WP_001223213.1
• Coordinates: 4670691..4670942 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS22440	4665801..4666823	-	1023	WP_001361374.1	ABC transporter permease	-
I2T83_RS22445	4666837..4668339	-	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
I2T83_RS22450	4668472..4669428	-	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
I2T83_RS22455	4669738..4670268	+	531	WP_000055075.1	inorganic diphosphatase	-
I2T83_RS22460	4670347..4670697	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
I2T83_RS22465	4670691..4670942	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
I2T83_RS22470	4671154..4671495	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
I2T83_RS22475	4671498..4675277	-	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T182098 WP_000239581.1 NZ_CP065136:c4670697-4670347 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T182098 NZ_CP086305:2412321-2412581 [Burkholderia ambifaria]
ATGCGCACGATTCACTTTTCGGATGCCCGCGGCAATCTGAAGACGGTCATCGACCAGGTCGTCGACGACGCGGACGTGAC
GCTCATCACACGTCGCGACGCGCCGAACGCCGTCATCATGTCGCAGGAATACTACGACAGCCTGATGGAGACGGTTCACC
TGCTCCGCTCGCCGGCCAACGTCGCCCACCTCGAACGCTCGATTGCGCAATTGCGCAAGGGCAAGGCGAAAGAGCACAAG
CTGGCGGAGGACGATGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT182098 WP_001223213.1 NZ_CP065136:c4670942-4670691 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT182098 NZ_CP086305:2412578-2412844 [Burkholderia ambifaria]
ATGAGCGTACGCCGCGTGCTCTTCACTTCCGACGCGCGGGACGACTACGTTTATTGGCAAGGGCAAGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAGCCCAACGGACGCCGTTCGAAGGCATCGGCAAACCCGAACCACTGAAGGCCA
ATCTGTCCGGTTTCTGGTCACGTCGCATCGACGATACGAACCGTCTCGTCTACGAAGCGGACGATCTTCAGATCAGCATC
GTGTCCTGCCGCTATCACTACGCGTAG

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7