Detailed information of TA system TA182095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4394396..4394883
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: I2T83_RS21120
• Protein ID: WP_001520461.1
• Coordinates: 4394396..4394647 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: I2T83_RS21125
• Protein ID: WP_001520458.1
• Coordinates: 4394650..4394883 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS21095	4390008..4391294	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
I2T83_RS21100	4391294..4392283	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
I2T83_RS21105	4392280..4393101	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
I2T83_RS21110	4393104..4393481	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
I2T83_RS21115	4393488..4394336	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
I2T83_RS21120	4394396..4394647	-	252	WP_001520461.1	CcdB family protein	Toxin
I2T83_RS21125	4394650..4394883	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
I2T83_RS21130	4394969..4395448	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
I2T83_RS21135	4395640..4397502	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
I2T83_RS21140	4397495..4398025	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
I2T83_RS21145	4398133..4399464	-	1332	WP_001520455.1	MFS transporter	-
I2T83_RS21150	4399523..4399810	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T182095 WP_001520461.1 NZ_CP065136:c4394647-4394396 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T182095 NZ_CP086305:c1990985-1990569 [Burkholderia ambifaria]
GTGTTTTTGATTGATACGAACGTCATCAGCGAGATCAGGAAGGGCAAGCGAACCAACCGCGGCGTGCGCGCGTTCTTCAG
GCAGGCGGAGGCCGACGCGAGCCCGCTTTACCTGTCGGTCGTGACCGTGGCCGAACTGCGGCGCGGCGTCGACCTGATCC
GTCATCGCGGCGACCATCCGCAGGCGTCGGCGCTCGAGGCATGGATGGCGACGATCCTGTCCGGCTATGCGTCGAACATA
CTGCCGGTCGATATCGAGGCCGCCCAGATGTGGGGCCATTTGCGCGTGCCCGATCCGGCGCACGCACTCGACCACCTGAT
CGCGGCCACCGCGCTGATCAACGATCTGACGGTCGTCACCCGCAATGTCGACGATTTCGCTCGCACCGGCGTCCGGCTGC
TGAACCCGTTCGATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT182095 WP_001520458.1 NZ_CP065136:c4394883-4394650 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT182095 NZ_CP086305:c1991208-1990972 [Burkholderia ambifaria]
ATGGCAAATCTTCTGGTGCGTAACGTGGATGACAGTATCGTTCAGAGCCTGCGTGAGCAGGCCGTGGCCAACGGCAGGAG
CGCCGAGGCCGAACATCGGGCCATTCTGGCCGAGGCGCTCGGCCGGCCGAAGCGACGGACATTCGCGCAAGTGCTGATGA
GCATGCCGGACGTCGGCGAAGACGCGGATTTCCAACGTGTTCAGGATTCCGGCGAGGTCAGGCGTGTTTTTGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4