Detailed information of TA system TA182093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4149032..4150721
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: I2T83_RS20000
• Protein ID: WP_001521912.1
• Coordinates: 4149032..4149952 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: I2T83_RS20005
• Protein ID: WP_001521911.1
• Coordinates: 4149939..4150721 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS19985	4145122..4146249	-	1128	WP_001521913.1	MFS transporter	-
I2T83_RS19990	4146391..4147449	-	1059	WP_122452191.1	alpha/beta hydrolase	-
I2T83_RS19995	4147714..4148598	+	885	WP_001119037.1	LysR family transcriptional regulator	-
I2T83_RS20000	4149032..4149952	-	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I2T83_RS20005	4149939..4150721	-	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
I2T83_RS20010	4151037..4154246	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
I2T83_RS20015	4154407..4154547	+	141	WP_001308408.1	ash family protein	-
I2T83_RS20020	4154593..4155063	+	471	WP_000201185.1	hypothetical protein	-
I2T83_RS20025	4155060..4155162	+	103	Protein_3925	helix-turn-helix domain-containing protein	-
I2T83_RS20030	4155254..4155412	+	159	Protein_3926	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T182093 WP_001521912.1 NZ_CP065136:c4149952-4149032 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T182093 NZ_CP086305:c1087278-1086979 [Burkholderia ambifaria]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGACTACTTCGAGGC
CTGTCTGCAGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGCGCGCGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCATGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAATTGCACGGCGCGAAGGCGCACGCGTGA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT182093 WP_001521911.1 NZ_CP065136:c4150721-4149939 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT182093 NZ_CP086305:c1087620-1087282 [Burkholderia ambifaria]
ATGGTTGACATGCTGCGATCCATGCCATACAGTGCACCTACATTCAGTATCCGGACCACCGACGTCTTCGACGCGTGGTT
TGCCGGCCTGCAGGATCGCGTCGCGAAGCGCCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTGTCGATCACGGGCCCGGCTATCGCGTCTACTACGTCAGGCGCGGA
ACGATATGGGTGATCCTGCTCTGCGGCGGCGACAAGTCGACGCAACAGGCGGACATCCGCGCCGCGCACGCGATGCTCGC
GCGGCTCGACCTGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829L165