Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3944306..3944924 Replicon chromosome
Accession NZ_CP065136
Organism Escherichia coli strain EE72d

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I2T83_RS19045 Protein ID WP_001291435.1
Coordinates 3944706..3944924 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I2T83_RS19040 Protein ID WP_000344800.1
Coordinates 3944306..3944680 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2T83_RS19030 3939395..3940588 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I2T83_RS19035 3940611..3943760 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
I2T83_RS19040 3944306..3944680 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I2T83_RS19045 3944706..3944924 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I2T83_RS19050 3945096..3945647 + 552 WP_001522034.1 maltose O-acetyltransferase -
I2T83_RS19055 3945763..3946233 + 471 WP_001490888.1 YlaC family protein -
I2T83_RS19060 3946397..3947947 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I2T83_RS19065 3947989..3948342 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I2T83_RS19075 3948721..3949032 + 312 WP_000409911.1 MGMT family protein -
I2T83_RS19080 3949063..3949635 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182092 WP_001291435.1 NZ_CP065136:3944706-3944924 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T182092 NZ_CP086305:30449-30820 [Burkholderia ambifaria]
ATGATTCTTGTCGACACTTCAGTGTGGATCGACCATCTGCGGGCCGGTGACGCAACGCTCGCCACATTGCTGGAAGCGGA
ACGCGTGTTGATCCATCCGTTCATCATCGGTGAGCTTGCGCTCGGCAGCATGCGCGATCGCCAGACCGTACTGGATGCGC
TGCGCGACCTTCCCGCCGCCGCGCCCGCGACGGACGACGAAGTCCAGCGCATGATCGATGTCGTACCGCTTCACGGGCTC
GGCATCGGTTACGTCGACGCCCATCTGCTTGCCTCGGTCAGACTGACCAGCGGCAGCAAGCTGTGGACGCGCGACCGGCG
CCTGCTGGCCGCAGCCGAGCGCCTGCAACTCGTCGCCCGCCCCGGTCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT182092 WP_000344800.1 NZ_CP065136:3944306-3944680 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT182092 NZ_CP086305:30255-30452 [Burkholderia ambifaria]
ATGCGCACCACCTTGTCCCTCGACGACGCACTGCTCGCCAAAGCGCAGCAATTAACCGGCGTCACCGAAAAATCGGCGCT
GGTGAGAGAAGCGCTGCGCGCTTTGATCGCGCGCGAAAGCGCACGACGACTTGCCCGCCTCGGCGGCACCGAGCCCGATC
TTGAGTCCGTTCCACGCCGCCCATCGGAGCCCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References