Detailed information of TA system TA182091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3522620..3523325
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I2T83_RS17195
• Protein ID: WP_000539521.1
• Coordinates: 3522620..3523006 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I2T83_RS17200
• Protein ID: WP_001280945.1
• Coordinates: 3522996..3523325 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS17175	3518624..3519250	+	627	WP_020233670.1	glutathione S-transferase GstB	-
I2T83_RS17180	3519247..3520362	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
I2T83_RS17185	3520473..3520856	-	384	WP_000497137.1	biofilm formation regulator BssR	-
I2T83_RS17190	3521069..3522394	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I2T83_RS17195	3522620..3523006	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I2T83_RS17200	3522996..3523325	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I2T83_RS17205	3523395..3524723	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
I2T83_RS17210	3524731..3527079	-	2349	WP_203135145.1	EAL domain-containing protein	-
I2T83_RS17215	3527257..3528168	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T182091 WP_000539521.1 NZ_CP065136:3522620-3523006 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T182091 NZ_CP086304:1754704-1755126 [Burkholderia ambifaria]
GTGACTACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCATCCGCGGTGTTATCCCGGTT
GCAGTCGTGCGTCAATGCACAGCACCGGATCGTCGTGTCGGCGATCACCTATGCCGAAATGCGGTTCGGCGCAGTCGGCA
GAAAGGCTTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTGTCGCGGCTGGATGGCGTGCTGTCATGGGACACCGCG
GCGGTGGATGCAACGGCCGCGATTCGCGTCGACCTGCCCGCACGTGGCACGCCGATCGGCGCGAACGACGCGTCGATCGC
CGGTCACGCGATCGCGGCCGGTGCCGTGCTTGTAACGAACAACAGTCGCGAATTCAGCCGTGTCGCGGGGTTGTCGCTCG
AAGACTGGGCAGCCTCGGCATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT182091 WP_001280945.1 NZ_CP065136:3522996-3523325 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT182091 NZ_CP086304:1754441-1754707 [Burkholderia ambifaria]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGTGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGTTACTGCTGCGTCCGGTGCGGCCGACGTGGCTGTCGTTCGCGAGTGAAC
CACGGGCCGATGCCGACTTCCTCCTCGAGCGCCCGACGGTCGTCGAACCCGGTCGTTTCGACCTGCCCAGCGCCGATGAC
GACGCACGGACGGAGTCGAACCGGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6