Detailed information of TA system TA182090

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3207793..3208588
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: I2T83_RS15700
• Protein ID: WP_000854914.1
• Coordinates: 3207793..3208167 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7UP42
• Locus tag: I2T83_RS15705
• Protein ID: WP_001280955.1
• Coordinates: 3208214..3208588 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS15660	3202797..3203288	-	492	WP_001308552.1	DUF1097 domain-containing protein	-
I2T83_RS15665	3203390..3203944	-	555	WP_001001921.1	molecular chaperone YcdY	-
I2T83_RS15670	3203968..3204705	-	738	WP_000283664.1	zinc-binding phosphatase	-
I2T83_RS15675	3204760..3205698	-	939	WP_001522839.1	glyoxylate/hydroxypyruvate reductase GhrA	-
I2T83_RS15685	3206169..3207011	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
I2T83_RS15690	3207096..3207293	-	198	WP_000839282.1	DUF957 domain-containing protein	-
I2T83_RS15695	3207305..3207796	-	492	WP_000976857.1	hypothetical protein	-
I2T83_RS15700	3207793..3208167	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
I2T83_RS15705	3208214..3208588	-	375	WP_001280955.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I2T83_RS15710	3208751..3208972	-	222	WP_001220314.1	DUF987 domain-containing protein	-
I2T83_RS15715	3209035..3209511	-	477	WP_001186738.1	RadC family protein	-
I2T83_RS15720	3209527..3210012	-	486	WP_000214398.1	antirestriction protein	-
I2T83_RS15725	3210103..3210921	-	819	WP_001234682.1	DUF945 domain-containing protein	-
I2T83_RS15730	3211261..3212331	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
I2T83_RS15735	3212328..3213233	-	906	WP_023149949.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T182090 WP_000854914.1 NZ_CP065136:c3208167-3207793 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T182090 NZ_CP086303:c893677-893378 [Burkholderia sp. B21-005]
ATGCCGATTGAGGCGCGTCTGGTGCGGCTGACGCCGCTGGCCGAGGCCGATCTCGAGGATATCTGGACCTACACCTTCGA
ACGCTGGTCGCTTGAGCAGGCCGAGCACTATGTGGGCGAGCTCGCCTCGGCGTTCGAGCGTCTCGCGCGGCGCGAGTGGA
GAGGGCGACCGAGTCGTGCGGGCAACGGCTATTGGCGCTACATGGTCGGTTCCCACGTGGTGTTCTATCGGGAAACGGCC
GAGAGCCTGGACGTGATTCGGGTATTGCATCAACGCATGGACGTGGACCGGCACCTGTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13767.59 Da
• Isoelectric Point: 6.6248

>AT182090 WP_001280955.1 NZ_CP065136:c3208588-3208214 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT182090 NZ_CP086303:c893912-893667 [Burkholderia sp. B21-005]
ATGGCACGGAACACTTCAATCTCGCTGGGAGACCACCTGACGCAGTTTGTCGACACGCAGGTCGCGTCCGGTCGCTATGG
CTCGGCCAGCGACGTGGTGCGGGCGGGGCTGCGCCTGCTCGAAACGCAGGAAACCGAGTTGCGGGCCCTGCAGGAGGCCT
TGAAGGCCGGCGAGGCCTCGGGCGAGCCGACTGCCTTTGACAGCCAGGCGTTCTTGGAGCGGATGCGGAAAAAGCATGCC
GATTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9P0D0