Detailed information of TA system TA182089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3161765..3162549
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I2T83_RS15425
• Protein ID: WP_000613626.1
• Coordinates: 3162055..3162549 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I2T83_RS15420
• Protein ID: WP_001110447.1
• Coordinates: 3161765..3162058 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS15410	3156915..3157874	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
I2T83_RS15415	3158447..3161632	+	3186	WP_001522867.1	ribonuclease E	-
I2T83_RS15420	3161765..3162058	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I2T83_RS15425	3162055..3162549	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I2T83_RS15430	3162644..3163597	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I2T83_RS15435	3163609..3165252	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
I2T83_RS15440	3165318..3166259	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I2T83_RS15445	3166259..3167356	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T182089 WP_000613626.1 NZ_CP065136:3162055-3162549 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T182089 NZ_CP086302:2314494-2315075 [Burkholderia sp. B21-005]
ATGAACCTCGACACGATCCACGCGCGGCTCGATTTCCTGCGCGCAGCCGAACGCCTGAAAGACGTGCTGCGCAGCGGCCA
CACGTCGACGGGCCGTCCCGAAAGCACGGCGGAGCACAGCTGGCGTCTCTGCCTGATGGCGCTCGTGTTCGCCGATGCGC
TGCCCGACGTCGACACGACGAAGCTGCTGAAATTGTGCGTCGTCCACGATCTCGGCGAAGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCATCCGGACAAGAGCACGCGCGAACGCGACGACCTGCTGACGCTCACGGCCGGGCTCGATCG
CGCGCTGCGCGACGAGATCGTCGCGCTGTGGGATGAATACGAGGCGGCCGAATCACCGGAAGCGCGCGCCGCGAAGGCGC
TCGACAAGCTGGAGACGATCCTGCAGCACAACCAGGGCAGCAATCCGCCCGACTTCGACTACGCGTTCAATCTCGGCTAC
GGGCGGCGCTACACCGACGCGACGCCGCTGTTCGGCGCGATCCGCGCGATCGTCGACGCGGACACGCAACGCCGGATCGA
CGCACGCGGCGACGGCGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT182089 WP_001110447.1 NZ_CP065136:3161765-3162058 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT182089 NZ_CP086302:2313828-2314385 [Burkholderia sp. B21-005]
ATGACGACCTCGCCGCTTCACACCCCGCCGCTCGGCCCGCAACTCAAGCGCTGGCGCACGCTGCGCCGCGTGAAGCAGAG
CCATGCGGCCGAATTGTTCGGTGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCATCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGCCGAGCGGCTGCTTGCCGCGCGTCTCGACTCGGCCGGCGACCACGCGCTCGCGCGGCTGATCGCGAGC
AGCACGGCCCGCATGCATCTCGTGTGCGACCTCACGCATCGTCTGCTCGCCTGTTCGCCGTCGCGCGCGGCCGAGTTCTC
ACGGCCGCTGCCGGCGTTGCTCGGTACCTCGCTGTGGCGCTATGCGTCGCCGGAAATCGTCCGCATGGAAGCCGCGCTCG
ACCCGCTCGGCTGGCACGATCGCGCGGGACCGCCGAGCGTCGAATTCGCGACCGGCACCAATGCGTCGCGCGTCGTGCCG
ATCCGCGGCAGCACCTGCCGGTGGACGCGCATGACGCTATCGGACGGCTCTGCCGCGCGGCTCGTCGAAACGCTGTAG

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6