Detailed information of TA system TA182088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2723389..2724027
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: I2T83_RS13150
• Protein ID: WP_001306887.1
• Coordinates: 2723851..2724027 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I2T83_RS13145
• Protein ID: WP_001270286.1
• Coordinates: 2723389..2723805 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS13125	2718541..2719482	-	942	WP_119163947.1	ABC transporter permease	-
I2T83_RS13130	2719483..2720496	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
I2T83_RS13135	2720514..2721659	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
I2T83_RS13140	2721904..2723310	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
I2T83_RS13145	2723389..2723805	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I2T83_RS13150	2723851..2724027	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I2T83_RS13155	2724249..2724479	+	231	WP_000494241.1	YncJ family protein	-
I2T83_RS13160	2724571..2726532	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I2T83_RS13165	2726605..2727141	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
I2T83_RS13170	2727194..2728405	+	1212	WP_023143671.1	BenE family transporter YdcO	-
I2T83_RS13175	2728513..2728971	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2728513..2728971	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T182088 WP_001306887.1 NZ_CP065136:c2724027-2723851 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T182088 NZ_CP086302:c2162106-2161687 [Burkholderia sp. B21-005]
ATGATCCTCGTCGATACCAACGTCATTTCCGAGCCGCTGCGGCGCGAGCCGAGTGCGGCCGTGATCGAATGGCTCGATGC
GCAGAATGTCGAGACGCTGTTTCTTGCCGCGATCAGCCTCGCGGAAATGCGCTTCGGCGTGGCCGCGTTGCAGGAAGGGC
GAAGGCGGGACTGGCTGCACCAAAGCATCGAGCATCGCGTCGTGCCGCTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCGGCCAGTAAAGCGTATGCGAGCCTTCGCGCGAAGGCTCGTGTTACGGGCAACGCGATGGCGTCTGCCGATGGATTGAT
CGCTGCAACGGCCGCGGCGAACGGCTTGATCGTGGCGACGCGTGACGTCGTGCCGTTCGAAGCGGCGGGCTTGCGCGTGA
TCGACCCGTGGGCCCGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT182088 WP_001270286.1 NZ_CP065136:c2723805-2723389 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT182088 NZ_CP086302:c2162342-2162103 [Burkholderia sp. B21-005]
ATGCCGGTTATTACCGTTCGAAATTTGCCCGACGAGGTGCATCGCGCGCTTCGCGTCCGTGCGGCGCAACATGGTCGCAG
TACCGAAGCGGAGGTGCGCGCCATCCTCGAGGAGGCCGTTCGGCCCGGCGGGCGGGCGAAGCTTGGGACGCTGCTGGCCG
AAATCGGGCGCGAGGCGGGTGGTGTCGATTTCGAGATTCCGCGCGAGAGCGCGCCAATCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76