Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2545719..2546245 Replicon chromosome
Accession NZ_CP065136
Organism Escherichia coli strain EE72d

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag I2T83_RS12260 Protein ID WP_000323025.1
Coordinates 2545958..2546245 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag I2T83_RS12255 Protein ID WP_000534858.1
Coordinates 2545719..2545958 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2T83_RS12230 2540981..2541595 + 615 WP_001265627.1 hypothetical protein -
I2T83_RS12235 2541592..2542620 + 1029 WP_001345551.1 DUF4868 domain-containing protein -
I2T83_RS12240 2543104..2544417 + 1314 WP_000589005.1 ISNCY family transposase -
I2T83_RS12245 2544854..2545186 - 333 WP_001301033.1 protein FlxA -
I2T83_RS12250 2545389..2545694 - 306 WP_001326990.1 hypothetical protein -
I2T83_RS12255 2545719..2545958 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
I2T83_RS12260 2545958..2546245 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
I2T83_RS12265 2546317..2546472 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
I2T83_RS12270 2546689..2546940 + 252 WP_000980994.1 hypothetical protein -
I2T83_RS12275 2547007..2547285 + 279 WP_012304870.1 hypothetical protein -
I2T83_RS12280 2547287..2548336 + 1050 WP_001265199.1 DUF968 domain-containing protein -
I2T83_RS12285 2548350..2549102 + 753 WP_001047135.1 antitermination protein -
I2T83_RS12290 2549380..2549469 - 90 WP_120795389.1 hypothetical protein -
I2T83_RS12295 2549524..2549736 - 213 WP_000087756.1 cold shock-like protein CspF -
I2T83_RS12300 2550037..2550252 + 216 WP_000066484.1 cold shock-like protein CspB -
I2T83_RS12305 2550703..2551161 + 459 WP_000526135.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2527295..2589701 62406
- flank IS/Tn - - 2550703..2551161 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T182087 WP_000323025.1 NZ_CP065136:2545958-2546245 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T182087 NZ_CP086302:c1974157-1973261 [Burkholderia sp. B21-005]
ATGAGTGTCATCGACAAACGTGAACGCGACCGCACCGCGGCGTTCGCCGCGCTGCTCGGGCAATTTCCCGAACAGCGCTG
CGCGGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGCGCGCAGCATGCGGGCCACGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCATCGTGGGTGTGCCAGCTCGACACGCTCGGCGCGTCGCGACGCGTGCTTGCGTGG
GATGCGCCCGGCTACGGCGTGTCGACCCCGGTGCACGGCGCGTCGCCGACCGCCGCCGATTACGCGGCGTCGCTGAACGC
GTGGCTCGAGGCACTCGGCATCGAACGCTGCGTGCTGGTCGGGCATTCGCTCGGCGCGATCATCGCGGGCGGCTTGTCCC
GCCTGATGCCCGCGCGGATCGCTGGCCTGCTGCTGATCTCGCCCGCGGGCGGCTACGGCCGCGCGCCCGCCGAAACGCGC
GACACGCGCCGCGACGCGCGCCTCGCGATGCTCGAGGCACTCGGTCCGGCCGGGCTCGCCGAGCAGCGCAGCGGCAACAT
GCTGTCGGGTTTTGCGAGCGAGGATGCGCGTGCGTGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCAC
AGGCCACGCATCTGCTCGCGAACGCCGATCTCGCGACCGATCTCGCCGGCTTTCGCGGCCGCACGGCCGTGGCCGTCGGC
GCGAACGACGCGATCACGCCGCCCGCCGCGTGCGAGCGGATCGCCGCGGCCGCGCAGGTCGAGCTGCAGGTGATTCCGCA
GGCCGGCCATGCCGGCTACGTGGAAGCGCCGGCCGTCTATTCGTCGCTCATCGACGCGTTCTGCCGTCAGTGCGACACGC
AGCGAGGTATGGCATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT182087 WP_000534858.1 NZ_CP065136:2545719-2545958 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT182087 NZ_CP086302:c1973264-1972407 [Burkholderia sp. B21-005]
ATGAGCGAACCGCTGCAACAACCAGAATCCGAGAGCACCAAGGCCGACGCCGGCTACGTGGTGCCGGGCCTCGAACGCGG
CCTGCGGATCCTCGCCGAATTCTCGCCGCGCGAGCCGGTGCTCGGCGCGCCGGAGCTGTCCAAGCGCCTCGGCATTCCGC
GCACGACGGTGTTCCGTCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGGGCGGACAAGGACCGCAACTACAAG
CTCGGCATCGCCGTGCTGCGGCTCGGCTTCGAATACCTGAGCTCGCTGGAGTTGACCGATCTCGGTCTGCCGGTGATCGA
GAGCCTGCGCGACGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTGGTGTTCGTCGCGAAGGCGC
AGTGCCAGTCGCCGGGGTTCAGCGCGATTCGCGTGAACGTCGGCACGCGGCTGCCCGCGCATGCGACGACCCACGGCCAC
GTGCTGATGGGCGACCTGTCGCTGAAGGAGCTGCGGGCGCTGTATCCGGAAGGCACGCTGAGCCGGATGACGAGCGCGAC
GCCGGAAACGGTCGACGCGCTGTACGAAGTGATCCGCGAGGATGCGCTGCGCGGCTACGGCATCAGCAATTCGTCGTTCG
AGCGCGGTATCTCGGTGGTCACGGCGCCGGTGCGCAACGACACGCAGAAGATCGTCGCGTGCATCACGGTGACGGTGCCG
CGGCCGGAAATCGACGCGGCGCTGATCGCCGACGGCCTGTTCGACAAGGTGCAGCGCGCGGCGGCGGAACTGTCGCGGCG
GCTCAATTACCGCTCGGATGACGAGCACACGTTTCTGAAGGCTTTAGGACTCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References