Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1024485..1025139 Replicon chromosome
Accession NZ_CP065136
Organism Escherichia coli strain EE72d

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I2T83_RS04940 Protein ID WP_000244781.1
Coordinates 1024732..1025139 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I2T83_RS04935 Protein ID WP_000354046.1
Coordinates 1024485..1024751 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2T83_RS04915 1020573..1022006 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
I2T83_RS04920 1022051..1022362 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
I2T83_RS04925 1022526..1023185 + 660 WP_000250269.1 hemolysin III family protein -
I2T83_RS04930 1023262..1024242 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
I2T83_RS04935 1024485..1024751 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I2T83_RS04940 1024732..1025139 + 408 WP_000244781.1 protein YgfX Toxin
I2T83_RS04945 1025179..1025700 - 522 WP_001521233.1 flavodoxin FldB -
I2T83_RS04950 1025812..1026708 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I2T83_RS04955 1026733..1027443 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I2T83_RS04960 1027449..1029182 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T182075 WP_000244781.1 NZ_CP065136:1024732-1025139 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T182075 NZ_CP086298:c853987-853580 [Burkholderia ambifaria]
ATGAAGTTTCTGCTGGATACCCATGCGGTAATCGCCATTCTGAAAGGCGAACAGGCCATGCTGGCCCGATTGCGTAGTCA
TCAACCTGCGGATTTCGGAATTCCCGCGATCGTCGCGCATGAACTCTATTACGGGGCGTACAAGAGTCAGAGAGCGGCAG
CGAATCTGGCACGCGTCGAGGGGCTTCAGTTCGAGGTCGTGTCGGTCGATGCGGAAGATGCGCAGCATGCCGGGGAAATC
CGTGCGCAACTGGCCGCGGTGGGAACACCGATCGGGCCATACGATGCATTGATCGCAGGACAGGCGCGAGCGCGACGCCT
GGTGCTTGTCACGCACAACACGCGCGAGTTTGAGCGGGTCCCGGAGCTACAGGTCGAGGATTGGCTCGCGGCGGCGCGCG
AAAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT182075 WP_000354046.1 NZ_CP065136:1024485-1024751 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT182075 NZ_CP086298:c854226-853984 [Burkholderia ambifaria]
ATGGAGACTGCCAAGATTTTCAAGCATGGTGGATCGCAGGCCGTCCGCTTGCCGAAGGATTTCCGCTTCGATACAACGGA
GGTCCGGATTCGTCGGCACGGCGCGTCTGTCATTCTCGAACCGGTGCCGCAAGACTGGGCATGGTTGACACCGTTGATCG
GCCCGGTTGACGCCGATTTCGAGGCCGCCGCGGCAACCGAGCCTGCCGCTCAAGAGCGTCCGGGGCTGGACGTGTTTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References