Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PHD(antitoxin)
Location 4936845..4937528 Replicon chromosome
Accession NZ_CP065047
Organism Mycobacterium kubicae strain JCM 13573

Toxin (Protein)


Gene name vapC Uniprot ID D5PEA4
Locus tag I2456_RS23060 Protein ID WP_007168487.1
Coordinates 4937121..4937528 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1X1XBB9
Locus tag I2456_RS23055 Protein ID WP_033711258.1
Coordinates 4936845..4937117 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2456_RS23035 4931971..4933032 + 1062 WP_007172499.1 IS256-like element IS1511 family transposase -
I2456_RS23040 4933101..4934150 + 1050 WP_007172498.1 tyrosine-type recombinase/integrase -
I2456_RS23045 4934147..4936090 + 1944 WP_007172497.1 tyrosine-type recombinase/integrase -
I2456_RS23050 4936134..4936355 + 222 Protein_4570 transposase -
I2456_RS23055 4936845..4937117 + 273 WP_033711258.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
I2456_RS23060 4937121..4937528 + 408 WP_007168487.1 type II toxin-antitoxin system VapC family toxin Toxin
I2456_RS23065 4937549..4937890 + 342 WP_007168486.1 hypothetical protein -
I2456_RS23070 4937951..4938256 + 306 WP_007168485.1 hypothetical protein -
I2456_RS23075 4938674..4939012 - 339 WP_080691075.1 hypothetical protein -
I2456_RS23080 4940005..4940175 + 171 Protein_4576 carnitinyl-CoA dehydratase -
I2456_RS23085 4940292..4940750 - 459 Protein_4577 IS30 family transposase -
I2456_RS23090 4940866..4942260 - 1395 WP_196763041.1 group II intron reverse transcriptase/maturase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4891286..5274661 383375
- flank IS/Tn - - 4931971..4933032 1061


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14464.55 Da        Isoelectric Point: 4.6697

>T182050 WP_007168487.1 NZ_CP065047:4937121-4937528 [Mycobacterium kubicae]
VIYLDTSALVKLIRIEVESDALADWLDERTETRWITSALAEVELPRAIRAVAPNGLSAVPSVLARLDRFEIDQVIRATAA
AYPDPALRSLDAIHLATAQIAASTAPLTAMVAYDSRLSDAARALGITIVTPGRNS

Download         Length: 408 bp

>T182050 NZ_CP086291:1695842-1696264 [Burkholderia ambifaria]
GTGACGACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCATCCGTGGTGTTGTCCCGGTT
GCAGTCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCGATCACCTATGCCGAGATGCGGTTCGGCGCAGTCGGCA
GAAAGGCTTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTTTCGCGGCTGGATGGCGTGCTGTCGTGGGATACCGCG
GCGGTCGATGCAACGACCGCGATTCGCGTCGACCTTGCCGCGCGTGGCACACCGATCGGCGCGAACGACGCGTCGATCGC
CGGTCATGCGATCGCGGCCGGTGCCGTGCTCGTAACGAACAACAGTCGCGAATTCAGCCGTGTCGCGGGGTTGTCGCTCG
AAGATTGGGCAGCCTCGGCCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9764.35 Da        Isoelectric Point: 8.7382

>AT182050 WP_033711258.1 NZ_CP065047:4936845-4937117 [Mycobacterium kubicae]
MSEVPLRMLNQETAKVLARVKRGEEITLTERGEVIARIIPATAGPLDALITAGRVQPATLAGPAPRPTVPMHDNLDAGAL
LQRLRAEERY

Download         Length: 273 bp

>AT182050 NZ_CP086291:1695579-1695845 [Burkholderia ambifaria]
ATGCGCACTGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGTGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGTTGCTGCTGCGTCCGGTGCGGCCGACGTGGCTGTCGTTCGCGACTGAAC
CACGGGCCGATGCCGACTTCCTCGTCGAACGCCCGACGGTCGTCGAACTTGGTCGTTTCGACCTGCGCGGCGCCGATGAC
GATGCACCGACGGAGTCGAACCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X1XAJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A1X1XBB9

References