Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/Polyketide_cyc2(toxin)
Location 4718554..4719193 Replicon chromosome
Accession NZ_CP065047
Organism Mycobacterium kubicae strain JCM 13573

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID A0A1X1X6Y1
Locus tag I2456_RS21980 Protein ID WP_068165541.1
Coordinates 4718554..4719009 (-) Length 152 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID A0A1X1X6A8
Locus tag I2456_RS21985 Protein ID WP_068028838.1
Coordinates 4719014..4719193 (-) Length 60 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2456_RS21955 4714698..4716092 + 1395 WP_196763041.1 group II intron reverse transcriptase/maturase -
I2456_RS21960 4716208..4716675 + 468 Protein_4352 IS30 family transposase -
I2456_RS21965 4716733..4717203 + 471 WP_085075676.1 VOC family protein -
I2456_RS21970 4717209..4717637 - 429 WP_068028828.1 hypothetical protein -
I2456_RS21975 4717701..4718477 - 777 WP_085075657.1 VOC family protein -
I2456_RS21980 4718554..4719009 - 456 WP_068165541.1 SRPBCC family protein Toxin
I2456_RS21985 4719014..4719193 - 180 WP_068028838.1 antitoxin Antitoxin
I2456_RS21990 4719267..4721636 - 2370 WP_085075658.1 HAD-IC family P-type ATPase -
I2456_RS21995 4721633..4723216 - 1584 WP_085075659.1 serine hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polyketide cyclase/dehydrase

(1-141)

Antitoxin

Antitoxin Rv0909/MT0933

(4-50)


Sequences

Toxin        


Download         Length: 152 a.a.        Molecular weight: 16307.95 Da        Isoelectric Point: 9.7517

>T182049 WP_068165541.1 NZ_CP065047:c4719009-4718554 [Mycobacterium kubicae]
MAKLSGSIDVPLPPEEAWNHASDLSRYKEWLTIHKVWRSPLPDVIEKGTVVESYVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVAPKGDGSVVSFDVHLGGPALFGPIGMVVAAALKTDIRESLQNFLAVFAGSERKPSG

Download         Length: 456 bp

>T182049 NZ_CP086291:153338-153739 [Burkholderia ambifaria]
ATGCCGCGCTATATGCTCGACACCAACATGTGCATCTATCTGATGAAGAATCAACCGGAGCAGGTCGCGAGGCGGTTCGC
GCAGTGTTACACCGGAGACGTCGTGATGTCGGCGATCACCTATGCCGAACTCGAGTACGGCGTTACCGTGTGCGCGAATC
CTGCCCGCGAGCGTCGCCATCTTGCCGCGCTGATTGACGATATTCCGGTTGCGCCGTTCGACGTCGCGGCTGCGCAAGCG
TATGGTCCGGTCCGCGAGGCAACCCGCGAGCGAAAGAAGGATCACCTCGACAAATTGATCGCCGCTCATGCGGTGTCGCT
CGATGTCGTCCTCGTGACCAACAACGAGCGGGATTTCGTCAGCTACCCGGGGTTGCGGTTGGAGAACTGGCTGAACGATT
AG

Antitoxin


Download         Length: 60 a.a.        Molecular weight: 6583.30 Da        Isoelectric Point: 5.0022

>AT182049 WP_068028838.1 NZ_CP065047:c4719193-4719014 [Mycobacterium kubicae]
MGFVDKAKDLLSQNADKVETAITKAGEFVDEKTQGKYTDTIHKVQEQARKVVDTGEQQN

Download         Length: 180 bp

>AT182049 NZ_CP086291:153102-153338 [Burkholderia ambifaria]
ATGCATACCACGAGGGTATTTCGGAACGGCAACTCGCAGGCCGTACGAATTCCTGCGGACCTTGCCTACGAGCGCAGCGA
TATCGAACTGGAAATCGAGCGCGTCGGTGACGAAATCCGGATTCGGCCGACGCGGCAGCCGCTGACCCACGTACTCGAGA
AATTTGCGAAATTCGGGCCGGATTTCATGGCCGAAGGACGAGGTGACCAGGAGCAGGCCGATCGCGAGGGTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X1X6Y1

Antitoxin

Source ID Structure
AlphaFold DB A0A1X1X6A8

References