Detailed information of TA system TA182048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3087704..3088694
• Replicon: chromosome
• Accession: NZ_CP065047
• Organism: Mycobacterium kubicae strain JCM 13573

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: I2456_RS14500
• Protein ID: WP_085074178.1
• Coordinates: 3087704..3088282 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A1X1XJY7
• Locus tag: I2456_RS14505
• Protein ID: WP_068033125.1
• Coordinates: 3088305..3088694 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2456_RS14460	3082751..3083299	-	549	WP_068033134.1	TetR/AcrR family transcriptional regulator	-
I2456_RS14465	3083411..3084157	+	747	WP_068033132.1	SDR family oxidoreductase	-
I2456_RS14470	3084167..3084625	-	459	WP_085074323.1	cupin domain-containing protein	-
I2456_RS14475	3084667..3085065	-	399	WP_085074322.1	VOC family protein	-
I2456_RS14480	3085110..3085958	-	849	WP_085074179.1	sigma-70 family RNA polymerase sigma factor	-
I2456_RS14485	3086038..3086274	+	237	WP_068033129.1	DUF1059 domain-containing protein	-
I2456_RS14490	3086432..3086860	+	429	WP_068033258.1	NAD(+)--rifampin ADP-ribosyltransferase	-
I2456_RS14495	3086913..3087593	-	681	WP_131813420.1	hypothetical protein	-
I2456_RS14500	3087704..3088282	-	579	WP_085074178.1	zeta toxin family protein	Toxin
I2456_RS14505	3088305..3088694	-	390	WP_068033125.1	hypothetical protein	Antitoxin
I2456_RS14510	3088940..3089911	+	972	WP_085074177.1	metal-dependent hydrolase	-
I2456_RS14515	3089908..3090096	+	189	WP_068033121.1	ferredoxin	-
I2456_RS14520	3090101..3091309	+	1209	WP_085074176.1	FAD-dependent oxidoreductase	-
I2456_RS14525	3091336..3092220	-	885	WP_085074175.1	helix-turn-helix transcriptional regulator	-
I2456_RS14530	3092291..3093097	-	807	WP_085074174.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-105)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21079.22 Da
• Isoelectric Point: 10.1277

>T182048 WP_085074178.1 NZ_CP065047:c3088282-3087704 [Mycobacterium kubicae]
VKRLDLVVGPNGAGKSTFIAFTLAPLLPRSVFVNADEIAKQRWPDDPAAHAYDAARIAANTRAKLIEAGVSFIAETVFSH
PSKLELIGSAHDAGYVIALHVMLIPEALAVERVKRRVRHGGHDVPESKIRERHRRLWRLVADAVEKCDTATIYDNSRIEG
PRIVAQLTAGTVIGAPTWPAWASTELSRRWPA

Nucleotide

• Length: 579 bp

>T182048 NZ_CP086288:c114349-113819 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13631.86 Da
• Isoelectric Point: 4.3810

>AT182048 WP_068033125.1 NZ_CP065047:c3088694-3088305 [Mycobacterium kubicae]
MANVADRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSSHQTASRRRVEAALAGDLDTNRLSDDEGLVFNAEI
SAALEESLAAAHYGDLLRAQGITTVALNDDGQIVEYQPDGTTSVVDVDG

Nucleotide

• Length: 390 bp

>AT182048 NZ_CP086288:c114622-114353 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1X1XJY7