Detailed information of TA system TA182045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4508324..4508916
• Replicon: chromosome
• Accession: NZ_CP065044
• Organism: Pectobacterium aroidearum strain L6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: I2D83_RS20090
• Protein ID: WP_181845790.1
• Coordinates: 4508662..4508916 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I2D83_RS20085
• Protein ID: WP_181845791.1
• Coordinates: 4508324..4508665 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2D83_RS20065	4503758..4504492	-	735	WP_205947201.1	type I-E CRISPR-associated protein Cas5/CasD	-
I2D83_RS20070	4504502..4505569	-	1068	WP_180778445.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
I2D83_RS20075	4505573..4506205	-	633	WP_205947202.1	type I-E CRISPR-associated protein Cse2/CasB	-
I2D83_RS20080	4506189..4507763	-	1575	WP_205947203.1	type I-E CRISPR-associated protein Cse1/CasA	-
I2D83_RS20085	4508324..4508665	-	342	WP_181845791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I2D83_RS20090	4508662..4508916	-	255	WP_181845790.1	type II toxin-antitoxin system HicA family toxin	Toxin
I2D83_RS20095	4509216..4511936	-	2721	WP_205947204.1	CRISPR-associated helicase Cas3'	-
I2D83_RS20100	4511973..4512524	-	552	WP_181829950.1	polymer-forming cytoskeletal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9554.01 Da
• Isoelectric Point: 11.0200

>T182045 WP_181845790.1 NZ_CP065044:c4508916-4508662 [Pectobacterium aroidearum]
MNKRHQKTLSDLFSRPVNGSIKWSDIESLFVALGAEIHEREGSRIAVLLQGEKKIFHRPHPRPTTDKGAVNSIRIWLESL
GVRP

Nucleotide

• Length: 255 bp

>T182045 NZ_CP086287:c4489652-4489368 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12358.99 Da
• Isoelectric Point: 4.5805

>AT182045 WP_181845791.1 NZ_CP065044:c4508665-4508324 [Pectobacterium aroidearum]
MINKLLIDGHPAVIAFDSEIEMFRGEFIGLNGGADFYAYSVDELKKEGATSLAIFLDECRRDGIEPYKSYSGKVTTRLTP
ERHAALAVAAQATGVSINELLNEGVDLVLERHS

Nucleotide

• Length: 342 bp

>AT182045 NZ_CP086287:c4489884-4489642 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA