Detailed information of TA system TA182043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4316122..4316648
• Replicon: chromosome
• Accession: NZ_CP065044
• Organism: Pectobacterium aroidearum strain L6

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: I2D83_RS19265
• Protein ID: WP_205947126.1
• Coordinates: 4316343..4316648 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: I2D83_RS19260
• Protein ID: WP_015855076.1
• Coordinates: 4316122..4316340 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2D83_RS19235	4312359..4313618	+	1260	WP_205947122.1	tyrosine-type recombinase/integrase	-
I2D83_RS19240	4313764..4313934	-	171	WP_164669135.1	hypothetical protein	-
I2D83_RS19245	4314075..4314416	+	342	WP_205947123.1	hypothetical protein	-
I2D83_RS19250	4314872..4315222	-	351	WP_205947124.1	hypothetical protein	-
I2D83_RS19255	4315320..4316063	+	744	WP_205947125.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
I2D83_RS19260	4316122..4316340	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I2D83_RS19265	4316343..4316648	+	306	WP_205947126.1	type II toxin-antitoxin system toxin CcdB	Toxin
I2D83_RS19270	4316962..4317171	+	210	WP_205947127.1	hypothetical protein	-
I2D83_RS19275	4317230..4318363	+	1134	WP_205554293.1	ThiF family adenylyltransferase	-
I2D83_RS19280	4318347..4319429	+	1083	WP_205947128.1	ThiF family adenylyltransferase	-
I2D83_RS19285	4319434..4321557	+	2124	WP_205947129.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4304362..4321557	17195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11764.67 Da
• Isoelectric Point: 4.6761

>T182043 WP_205947126.1 NZ_CP065044:4316343-4316648 [Pectobacterium aroidearum]
MQFIVYEYKRASHYKMFVDVQSDIVDTPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEAI
MDLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T182043 NZ_CP086287:3801513-3801731 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT182043 WP_015855076.1 NZ_CP065044:4316122-4316340 [Pectobacterium aroidearum]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT182043 NZ_CP086287:3801111-3801485 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46