Detailed information of TA system TA182041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3956810..3957594
• Replicon: chromosome
• Accession: NZ_CP065044
• Organism: Pectobacterium aroidearum strain L6

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I2D83_RS17660
• Protein ID: WP_205947013.1
• Coordinates: 3956810..3957304 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: I2D83_RS17665
• Protein ID: WP_205947014.1
• Coordinates: 3957301..3957594 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2D83_RS17640	3952030..3952248	+	219	WP_205947011.1	type II toxin-antitoxin system antitoxin CcdA	-
I2D83_RS17645	3952251..3952444	+	194	Protein_3450	CcdB family protein	-
I2D83_RS17655	3954025..3956538	-	2514	WP_205947012.1	ATP-binding protein	-
I2D83_RS17660	3956810..3957304	-	495	WP_205947013.1	GNAT family N-acetyltransferase	Toxin
I2D83_RS17665	3957301..3957594	-	294	WP_205947014.1	DUF1778 domain-containing protein	Antitoxin
I2D83_RS17670	3957803..3958759	-	957	WP_205947015.1	AraC family transcriptional regulator	-
I2D83_RS17675	3958875..3960062	+	1188	WP_205947016.1	MFS transporter	-
I2D83_RS17680	3960191..3960613	-	423	WP_039283843.1	MarR family transcriptional regulator	-
I2D83_RS17685	3960774..3961793	+	1020	WP_205947017.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3951427..3972750	21323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17665.49 Da
• Isoelectric Point: 7.2922

>T182041 WP_205947013.1 NZ_CP065044:c3957304-3956810 [Pectobacterium aroidearum]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMLLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T182041 NZ_CP086287:867267-867674 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10892.43 Da
• Isoelectric Point: 10.1968

>AT182041 WP_205947014.1 NZ_CP065044:c3957594-3957301 [Pectobacterium aroidearum]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLTRLDQAPSPN
AALRKTMQTSAPWEQKK

Nucleotide

• Length: 294 bp

>AT182041 NZ_CP086287:867020-867286 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA