Detailed information of TA system TA182038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1809231..1809784
• Replicon: chromosome
• Accession: NZ_CP065044
• Organism: Pectobacterium aroidearum strain L6

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: I2D83_RS08075
• Protein ID: WP_205947862.1
• Coordinates: 1809470..1809784 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: I2D83_RS08070
• Protein ID: WP_205947861.1
• Coordinates: 1809231..1809467 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2D83_RS08045	1804378..1804872	-	495	WP_205947857.1	threonine transporter	-
I2D83_RS08050	1804869..1805957	-	1089	WP_205947858.1	hypothetical protein	-
I2D83_RS08055	1805954..1806949	-	996	WP_205947859.1	restriction endonuclease	-
I2D83_RS08060	1807500..1807741	-	242	Protein_1566	hypothetical protein	-
I2D83_RS08065	1807965..1808912	+	948	WP_205947860.1	DUF1738 domain-containing protein	-
I2D83_RS08070	1809231..1809467	+	237	WP_205947861.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
I2D83_RS08075	1809470..1809784	+	315	WP_205947862.1	CcdB family protein	Toxin
I2D83_RS08080	1810477..1814178	+	3702	WP_205947863.1	RNA-directed DNA polymerase	-
I2D83_RS08085	1814187..1814621	+	435	WP_205947864.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1788100..1869688	81588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11549.50 Da
• Isoelectric Point: 7.9859

>T182038 WP_205947862.1 NZ_CP065044:1809470-1809784 [Pectobacterium aroidearum]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPIQALG
AVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T182038 NZ_CP086287:34610-34972 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8930.00 Da
• Isoelectric Point: 6.4550

>AT182038 WP_205947861.1 NZ_CP065044:1809231-1809467 [Pectobacterium aroidearum]
MTAKQRNTQSVTMSVERALLVRAREAGINLSATLTTALDAELRCHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT182038 NZ_CP086287:34959-35258 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA