Detailed information of TA system TA182023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1797186..1797835
• Replicon: chromosome
• Accession: NZ_CP065039
• Organism: Proteus mirabilis strain XH1653

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: I1A52_RS08315
• Protein ID: WP_012368534.1
• Coordinates: 1797416..1797835 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: I1A52_RS08310
• Protein ID: WP_012368535.1
• Coordinates: 1797186..1797419 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I1A52_RS08300 (I1A52_08280)	1792831..1794240	-	1410	WP_004246801.1	glutamate--ammonia ligase	-
I1A52_RS08305 (I1A52_08285)	1794600..1796435	+	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
I1A52_RS08310 (I1A52_08290)	1797186..1797419	+	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I1A52_RS08315 (I1A52_08295)	1797416..1797835	+	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
I1A52_RS08320 (I1A52_08300)	1798060..1798383	+	324	WP_049208253.1	hypothetical protein	-
I1A52_RS08325 (I1A52_08305)	1798355..1798777	+	423	WP_202611289.1	hypothetical protein	-
I1A52_RS08330 (I1A52_08310)	1799431..1800048	+	618	WP_004246809.1	glucose-1-phosphatase	-
I1A52_RS08335 (I1A52_08315)	1800129..1800566	+	438	WP_004246810.1	D-aminoacyl-tRNA deacylase	-
I1A52_RS08340 (I1A52_08320)	1800590..1801504	+	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
I1A52_RS08345 (I1A52_08325)	1801680..1802558	+	879	WP_049203255.1	23S rRNA pseudouridine(2604) synthase RluF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T182023 WP_012368534.1 NZ_CP065039:1797416-1797835 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T182023 NZ_CP086261:32103-32504 [Escherichia coli]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT182023 WP_012368535.1 NZ_CP065039:1797186-1797419 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT182023 NZ_CP086261:31849-32079 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0