Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3003065..3003642 Replicon chromosome
Accession NZ_CP065037
Organism Leifsonia sp. ZF2019

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag IT072_RS14775 Protein ID WP_223357617.1
Coordinates 3003065..3003424 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag IT072_RS14780 Protein ID WP_223357618.1
Coordinates 3003421..3003642 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IT072_RS14740 (IT072_14655) 2998558..2999637 - 1080 WP_223357610.1 hypothetical protein -
IT072_RS14745 (IT072_14660) 2999693..3000355 - 663 WP_223357611.1 GNAT family protein -
IT072_RS14750 (IT072_14665) 3000424..3001377 - 954 WP_223357612.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
IT072_RS14755 (IT072_14670) 3001437..3002021 + 585 WP_223357613.1 5-formyltetrahydrofolate cyclo-ligase -
IT072_RS14760 (IT072_14675) 3002062..3002367 + 306 WP_223357614.1 zinc ribbon domain-containing protein -
IT072_RS14765 (IT072_14680) 3002411..3002848 + 438 WP_223357615.1 large conductance mechanosensitive channel protein MscL -
IT072_RS14770 (IT072_14685) 3002860..3003045 - 186 WP_223357616.1 hypothetical protein -
IT072_RS14775 (IT072_14690) 3003065..3003424 - 360 WP_223357617.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IT072_RS14780 (IT072_14695) 3003421..3003642 - 222 WP_223357618.1 antitoxin Antitoxin
IT072_RS14785 (IT072_14700) 3003671..3007432 - 3762 WP_223357619.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13135.99 Da        Isoelectric Point: 6.8712

>T182016 WP_223357617.1 NZ_CP065037:c3003424-3003065 [Leifsonia sp. ZF2019]
VRRGEVYDVQLDPVVEGEAARLRPCIVVSNEGVNASVTRRGRGVLAFVPLTSNVANVHEFQVAVDDPEDLALMGLDRASK
IQAEQVRAIGHPRIGRYRGVTPAWIMHQVDDALRFHLSL

Download         Length: 360 bp

>T182016 NZ_CP086259:c4574642-4574292 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8059.78 Da        Isoelectric Point: 3.8962

>AT182016 WP_223357618.1 NZ_CP065037:c3003642-3003421 [Leifsonia sp. ZF2019]
MTKLSVSLSEEDVAYLDVVAADFAGNRSAAIHRLIRLKRELDAEGAYAQAFDEWEASGEQELWDQTAGDGLEE

Download         Length: 222 bp

>AT182016 NZ_CP086259:c4574887-4574636 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References