Detailed information of TA system TA182015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1437101..1437642
• Replicon: chromosome
• Accession: NZ_CP065037
• Organism: Leifsonia sp. ZF2019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IT072_RS07065
• Protein ID: WP_223360246.1
• Coordinates: 1437376..1437642 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IT072_RS07060
• Protein ID: WP_223360245.1
• Coordinates: 1437101..1437379 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT072_RS07035 (IT072_07000)	1432758..1433441	+	684	WP_223360240.1	response regulator transcription factor	-
IT072_RS07040 (IT072_07005)	1433434..1434879	+	1446	WP_223360241.1	HAMP domain-containing sensor histidine kinase	-
IT072_RS07045 (IT072_07010)	1434904..1435512	+	609	WP_223360242.1	DUF5666 domain-containing protein	-
IT072_RS07050 (IT072_07015)	1435573..1436244	+	672	WP_223360243.1	FCD domain-containing protein	-
IT072_RS07055 (IT072_07020)	1436241..1437050	+	810	WP_223360244.1	ATP-binding cassette domain-containing protein	-
IT072_RS07060 (IT072_07025)	1437101..1437379	+	279	WP_223360245.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IT072_RS07065 (IT072_07030)	1437376..1437642	+	267	WP_223360246.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IT072_RS07070 (IT072_07035)	1437677..1438642	-	966	WP_223360247.1	NAD(P)/FAD-dependent oxidoreductase	-
IT072_RS07075 (IT072_07040)	1438708..1439322	+	615	WP_223360248.1	XRE family transcriptional regulator	-
IT072_RS07080 (IT072_07045)	1439334..1440125	+	792	WP_223360249.1	alpha/beta fold hydrolase	-
IT072_RS07085 (IT072_07050)	1440133..1440501	-	369	WP_223360250.1	VOC family protein	-
IT072_RS07090 (IT072_07055)	1440565..1441017	+	453	WP_223360251.1	MarR family transcriptional regulator	-
IT072_RS07095 (IT072_07060)	1441062..1442453	-	1392	WP_223360252.1	DUF2252 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10241.81 Da
• Isoelectric Point: 9.8737

>T182015 WP_223360246.1 NZ_CP065037:1437376-1437642 [Leifsonia sp. ZF2019]
MTYEVIFASTAVRDLERIPPRIVPAVIEFAYGDLARNPRRVGKALERELSGLFSARRGPYRILYEIQDEEVRVLVVRVDH
RADAYRPR

Nucleotide

• Length: 267 bp

>T182015 NZ_CP086259:4097331-4097609 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10123.37 Da
• Isoelectric Point: 4.5686

>AT182015 WP_223360245.1 NZ_CP065037:1437101-1437379 [Leifsonia sp. ZF2019]
MQTLPISAVKARLSELVDSASHTREEVAITVNGSPAAMLIGIDEWEELQERLHWLSTGDDLVARLAEARREVESGGGLGE
DEIRARFGVPRR

Nucleotide

• Length: 279 bp

>AT182015 NZ_CP086259:4097068-4097328 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA