Detailed information of TA system TA182014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 4814615..4815146
• Replicon: chromosome
• Accession: NZ_CP065036
• Organism: Pectobacterium parmentieri strain PB20

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: PB20LOC_RS22010
• Protein ID: WP_103808553.1
• Coordinates: 4814859..4815146 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: PB20LOC_RS22005
• Protein ID: WP_103808552.1
• Coordinates: 4814615..4814869 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PB20LOC_RS21980	4809833..4810423	-	591	WP_011091952.1	DnaA initiator-associating protein DiaA	-
PB20LOC_RS21985	4810465..4810821	-	357	WP_012822117.1	YraN family protein	-
PB20LOC_RS21990	4810818..4812836	-	2019	WP_014698522.1	penicillin-binding protein activator	-
PB20LOC_RS21995	4812898..4813785	+	888	WP_014698523.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
PB20LOC_RS22005	4814615..4814869	+	255	WP_103808552.1	plasmid stabilization protein	Antitoxin
PB20LOC_RS22010	4814859..4815146	+	288	WP_103808553.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PB20LOC_RS22015	4815328..4816116	+	789	WP_033072300.1	4,5-DOPA dioxygenase extradiol	-
PB20LOC_RS22020	4816204..4817364	-	1161	WP_103808554.1	glutathionylspermidine synthase family protein	-
PB20LOC_RS22025	4817376..4818044	-	669	WP_012822126.1	DUF1190 family protein	-
PB20LOC_RS22030	4818328..4819719	-	1392	WP_012822127.1	outer membrane channel protein TolC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.89 Da
• Isoelectric Point: 10.7148

>T182014 WP_103808553.1 NZ_CP065036:4814859-4815146 [Pectobacterium parmentieri]
MTYKLKFVPSAMKEWKKLGHPVREQFKKKLVERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQDETITVMVMGVGK
REGSQVYSDTQGRSA

Nucleotide

• Length: 288 bp

>T182014 NZ_CP086259:3922102-3922644 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9284.50 Da
• Isoelectric Point: 3.7947

>AT182014 WP_103808552.1 NZ_CP065036:4814615-4814869 [Pectobacterium parmentieri]
MAFNILTNTVASITELKRDPMGTVRSAEGETIAILNRNEPAFYCVPPAMFAYLMELAEDAELGRTVDDRMSELADAEEIS
LDDL

Nucleotide

• Length: 255 bp

>AT182014 NZ_CP086259:3921807-3922118 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA