Detailed information of TA system TA182012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3091325..3091851
• Replicon: chromosome
• Accession: NZ_CP065036
• Organism: Pectobacterium parmentieri strain PB20

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: PB20LOC_RS14030
• Protein ID: WP_103808718.1
• Coordinates: 3091546..3091851 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0SK21
• Locus tag: PB20LOC_RS14025
• Protein ID: WP_012773205.1
• Coordinates: 3091325..3091543 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PB20LOC_RS14000	3087548..3088807	+	1260	WP_103808713.1	integrase arm-type DNA-binding domain-containing protein	-
PB20LOC_RS14005	3088914..3089108	-	195	WP_103808714.1	DUF3950 domain-containing protein	-
PB20LOC_RS14010	3089294..3089635	+	342	WP_103808715.1	hypothetical protein	-
PB20LOC_RS14015	3090080..3090403	-	324	WP_162940689.1	hypothetical protein	-
PB20LOC_RS14020	3090523..3091266	+	744	WP_103808717.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
PB20LOC_RS14025	3091325..3091543	+	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
PB20LOC_RS14030	3091546..3091851	+	306	WP_103808718.1	type II toxin-antitoxin system toxin CcdB	Toxin
PB20LOC_RS14035	3092203..3093933	+	1731	WP_103808719.1	hypothetical protein	-
PB20LOC_RS14040	3094208..3095404	+	1197	WP_103808720.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3087381..3101866	14485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11786.68 Da
• Isoelectric Point: 4.6873

>T182012 WP_103808718.1 NZ_CP065036:3091546-3091851 [Pectobacterium parmentieri]
MQFIVYEYKRASHYKIFVDVQSDIVETPKRRMVIPLIEAHHLSEKVNKTLFPLLRIDGEDYRLMTTELSSVPVEVIGEVI
VDLSDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T182012 NZ_CP086259:3176666-3177043 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCTCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGCATTTCACTGTGTGACGAGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8201.16 Da
• Isoelectric Point: 7.2038

>AT182012 WP_012773205.1 NZ_CP065036:3091325-3091543 [Pectobacterium parmentieri]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT182012 NZ_CP086259:3176208-3176576 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: E0SK21