Detailed information of TA system TA182006

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 127417..128131
• Replicon: chromosome
• Accession: NZ_CP065036
• Organism: Pectobacterium parmentieri strain PB20

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: PB20LOC_RS00685
• Protein ID: WP_103808670.1
• Coordinates: 127811..128131 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: PB20LOC_RS00680
• Protein ID: WP_103808669.1
• Coordinates: 127417..127752 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PB20LOC_RS00650	123137..124069	+	933	WP_103808665.1	hypothetical protein	-
PB20LOC_RS00655	124164..125051	+	888	WP_103808666.1	50S ribosome-binding GTPase	-
PB20LOC_RS00660	125235..125393	+	159	WP_155242703.1	hypothetical protein	-
PB20LOC_RS00665	125540..126373	+	834	WP_103808667.1	hypothetical protein	-
PB20LOC_RS00670	126431..126781	+	351	WP_005974477.1	hypothetical protein	-
PB20LOC_RS00675	126904..127377	+	474	WP_103808668.1	DNA repair protein RadC	-
PB20LOC_RS00680	127417..127752	+	336	WP_103808669.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PB20LOC_RS00685	127811..128131	+	321	WP_103808670.1	TA system toxin CbtA family protein	Toxin
PB20LOC_RS00690	128249..129082	+	834	Protein_136	DUF4942 domain-containing protein	-
PB20LOC_RS00695	129192..129440	+	249	WP_103808671.1	ribbon-helix-helix domain-containing protein	-
PB20LOC_RS00700	129444..129719	+	276	WP_039517502.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PB20LOC_RS00705	129767..130279	+	513	WP_103808672.1	AAA family ATPase	-
PB20LOC_RS00710	130586..131706	-	1121	Protein_140	IS3 family transposase	-
PB20LOC_RS00715	131778..132946	+	1169	WP_103808047.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtB / hcp	107012..249782	142770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12049.71 Da
• Isoelectric Point: 4.5971

>T182006 WP_103808670.1 NZ_CP065036:127811-128131 [Pectobacterium parmentieri]
MQTSPAIPPREDNPCPSPIAAWQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDAGITLVNAVNFLVEKYELVRIDRDGF
NWQEQSPFLTAVDVLRARRANGLLKA

Nucleotide

• Length: 321 bp

>T182006 NZ_CP086259:2009376-2009750 [Escherichia coli]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCATGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12529.30 Da
• Isoelectric Point: 5.8292

>AT182006 WP_103808669.1 NZ_CP065036:127417-127752 [Pectobacterium parmentieri]
MSLSDTPEWGLKSAITPRFGARLVQEGNRLHYLADRVSFIGTFSKTEAQSLERCFPELIKLLEQKLRMGELNPRQQGCIT
LYCNEWTCEADTLGSFGYVYIAIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT182006 NZ_CP086259:2008932-2009285 [Escherichia coli]
GTGTCAGACACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGCGC
CCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATG
CGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTCACGCTATATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCT
GGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA