Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 56866..57602 Replicon plasmid unnamed
Accession NZ_CP065035
Organism Klebsiella pneumoniae strain 2016-1400

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag GOY40_RS25080 Protein ID WP_003026803.1
Coordinates 57120..57602 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag GOY40_RS25075 Protein ID WP_003026799.1
Coordinates 56866..57132 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GOY40_RS25030 52274..52624 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
GOY40_RS25035 52740..53708 + 969 WP_022649395.1 IS5 family transposase -
GOY40_RS25040 54048..54317 + 270 WP_004152102.1 hypothetical protein -
GOY40_RS25045 54305..54880 + 576 WP_004152103.1 hypothetical protein -
GOY40_RS25050 54911..55405 + 495 WP_004152104.1 DNA-binding protein -
GOY40_RS25055 55449..55817 + 369 WP_004152105.1 hypothetical protein -
GOY40_RS25060 55851..56054 + 204 WP_004152106.1 hemolysin expression modulator Hha -
GOY40_RS25065 56103..56360 + 258 WP_004152107.1 hypothetical protein -
GOY40_RS25070 56436..56690 + 255 WP_004152108.1 hypothetical protein -
GOY40_RS25075 56866..57132 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
GOY40_RS25080 57120..57602 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
GOY40_RS25085 57763..59017 - 1255 Protein_61 IS3 family transposase -
GOY40_RS25090 59105..60067 - 963 WP_004152113.1 M48 family metalloprotease -
GOY40_RS25095 60054..60803 - 750 WP_009483782.1 phosphate-starvation-inducible PsiE family protein -
GOY40_RS25100 61041..61238 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-3 / blaTEM-1B - 1..191744 191744
- inside IScluster/Tn - - 52740..58790 6050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T182004 WP_003026803.1 NZ_CP065035:57120-57602 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T182004 NZ_CP086259:1949164-1949415 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT182004 WP_003026799.1 NZ_CP065035:56866-57132 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT182004 NZ_CP086259:1949412-1949666 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References