Detailed information of TA system TA182001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4443121..4443931
• Replicon: chromosome
• Accession: NZ_CP065034
• Organism: Klebsiella pneumoniae strain 2016-1400

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GOY40_RS21730
• Protein ID: WP_087788033.1
• Coordinates: 4443121..4443654 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: GOY40_RS21735
• Protein ID: WP_002887278.1
• Coordinates: 4443665..4443931 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOY40_RS21725	4441952..4443073	+	1122	WP_004214138.1	cupin domain-containing protein	-
GOY40_RS21730	4443121..4443654	-	534	WP_087788033.1	GNAT family N-acetyltransferase	Toxin
GOY40_RS21735	4443665..4443931	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
GOY40_RS21740	4444034..4445467	-	1434	WP_087788034.1	Cu(+)/Ag(+) sensor histidine kinase	-
GOY40_RS21745	4445457..4446140	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
GOY40_RS21750	4446312..4447697	+	1386	WP_087788035.1	efflux transporter outer membrane subunit	-
GOY40_RS21755	4447715..4448059	+	345	WP_087788039.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19844.67 Da
• Isoelectric Point: 5.2614

>T182001 WP_087788033.1 NZ_CP065034:c4443654-4443121 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRFLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T182001 NZ_CP086259:1027879-1028286 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT182001 WP_002887278.1 NZ_CP065034:c4443931-4443665 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT182001 NZ_CP086259:1027632-1027898 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1