Detailed information of TA system TA181998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2510268..2511856
• Replicon: chromosome
• Accession: NZ_CP065034
• Organism: Klebsiella pneumoniae strain 2016-1400

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: GOY40_RS12355
• Protein ID: WP_023301940.1
• Coordinates: 2510268..2511590 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GOY40_RS12360
• Protein ID: WP_087787682.1
• Coordinates: 2511590..2511856 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOY40_RS12340	2505324..2507825	+	2502	WP_087787686.1	fimbrial biogenesis outer membrane usher protein	-
GOY40_RS12345	2507813..2508817	+	1005	WP_087787685.1	type 1 fimbrial protein	-
GOY40_RS12350	2508959..2510251	+	1293	WP_087787684.1	glycoside hydrolase family 10 protein	-
GOY40_RS12355	2510268..2511590	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
GOY40_RS12360	2511590..2511856	-	267	WP_087787682.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GOY40_RS12365	2512121..2512447	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
GOY40_RS12370	2512444..2512944	-	501	WP_023282573.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GOY40_RS12375	2512922..2514301	-	1380	WP_087787681.1	purine permease	-
GOY40_RS12380	2514647..2515801	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
GOY40_RS12385	2515782..2516705	-	924	WP_080882515.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE / sciN/tssJ / tssG / tssF / icmF/tssM / KPHS_23120 / tle1 / tli1 / tli1 / tli1 / vgrG/tssI / clpV/tssH / hcp/tssD / dotU/tssL / vasE/tssK / vipB/tssC / vipA/tssB	2341323..2865046	523723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T181998 WP_023301940.1 NZ_CP065034:c2511590-2510268 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T181998 NZ_CP086259:354867-355097 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9950.28 Da
• Isoelectric Point: 7.1999

>AT181998 WP_087787682.1 NZ_CP065034:c2511856-2511590 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNDKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT181998 NZ_CP086259:355094-355453 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A486S9S0