Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 317798..318384 Replicon chromosome
Accession NZ_CP065034
Organism Klebsiella pneumoniae strain 2016-1400

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag GOY40_RS01505 Protein ID WP_002920800.1
Coordinates 318016..318384 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag GOY40_RS01500 Protein ID WP_004174006.1
Coordinates 317798..318019 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GOY40_RS01480 313955..314881 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GOY40_RS01485 314878..316155 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
GOY40_RS01490 316152..316919 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GOY40_RS01495 316921..317634 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
GOY40_RS01500 317798..318019 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GOY40_RS01505 318016..318384 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GOY40_RS01510 318657..319973 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
GOY40_RS01515 320080..320967 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
GOY40_RS01520 320964..321809 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
GOY40_RS01525 321811..322881 + 1071 WP_087788088.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 314878..326409 11531


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T181987 WP_002920800.1 NZ_CP065034:318016-318384 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T181987 NZ_CP086253:4322184-4322471 [Pectobacterium aquaticum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGCTAAAACTCGATAAAGCTATCCAACAGCAATTCGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTGCGGCAAAGTTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCGTCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTGGTTATCGCCGTGGTGGCGGTAGGCAAA
CGTGAGCGCAGCGGCGTTTATCATCTGGCGAGTGAGCGAATGAGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT181987 WP_004174006.1 NZ_CP065034:317798-318019 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT181987 NZ_CP086253:4321936-4322187 [Pectobacterium aquaticum]
ATGCCGAATATTATATTGAGTGATACCAGTGCTAGCGTCAGTGAACTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
TGATGGCCTGCCTGTCGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGATTAAGCTGATCGGTGAACGTAAAAACCAGCCGCTGGTTGAGGTGGATTTGGAT
AGTTTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References