Detailed information of TA system TA181969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 5046199..5047784
• Replicon: chromosome
• Accession: NZ_CP065031
• Organism: Pectobacterium brasiliense strain F126

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: F126LOC_RS22250
• Protein ID: WP_119870763.1
• Coordinates: 5046465..5047784 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F126LOC_RS22245
• Protein ID: WP_014917131.1
• Coordinates: 5046199..5046465 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F126LOC_RS22215	5041234..5041893	+	660	WP_014917137.1	DUF799 domain-containing protein	-
F126LOC_RS22220	5042016..5043518	-	1503	WP_119870760.1	MFS transporter	-
F126LOC_RS22225	5043622..5044173	+	552	WP_119870761.1	TetR/AcrR family transcriptional regulator	-
F126LOC_RS22230	5044204..5044614	-	411	WP_094431545.1	helix-turn-helix transcriptional regulator	-
F126LOC_RS22235	5044757..5045608	+	852	WP_119870762.1	SDR family oxidoreductase	-
F126LOC_RS22240	5045737..5046003	+	267	Protein_4343	alpha/beta hydrolase	-
F126LOC_RS22245	5046199..5046465	+	267	WP_014917131.1	helix-turn-helix domain-containing protein	Antitoxin
F126LOC_RS22250	5046465..5047784	+	1320	WP_119870763.1	type II toxin-antitoxin system HipA family toxin	Toxin
F126LOC_RS22255	5047817..5049181	-	1365	WP_119870764.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
F126LOC_RS22260	5049311..5050945	-	1635	WP_010281240.1	membrane protein insertase YidC	-
F126LOC_RS22265	5050948..5051205	-	258	WP_010281237.1	membrane protein insertion efficiency factor YidD	-
F126LOC_RS22270	5051169..5051528	-	360	WP_010281235.1	ribonuclease P protein component	-
F126LOC_RS22275	5051547..5051687	-	141	WP_005976668.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49397.67 Da
• Isoelectric Point: 6.6897

>T181969 WP_119870763.1 NZ_CP065031:5046465-5047784 [Pectobacterium brasiliense]
MAALDVYMNGYRVGILTKTGSGAHHFSYDTNWLGLLGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
TDSTQPFDLLAKVGQDSVGALQLVPQGTPVHDIKKIEYKTLSEQQLENILTGYLSDAPLGMIDTEDDFRISIAGAQEKTA
LLYLDDRWCLPLNVTPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSVYPERDRYRFMQTQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAKVVGFARESMENILAEFAQTV
DTVMTTVGSQLPVDFPTHISEAILAGLQARSRRLMSGWE

Nucleotide

• Length: 1320 bp

>T181969 NZ_CP086250:c218505-218248 [Clostridium estertheticum]
ATGAATAAGATTTTTTCAGAGGTATCATGGGAGCATTATGTATATTGGCAGTCAGAGGATAAAAAAATATTAAGAAAAAT
AAATGAACTTATAAAAGATATTGAAAGAAATGGAAATGAAGGTCTAGGAAAGCCTGAAGCATTAAAACATGAGTTGGTTG
GTTATTGGAGTCGAAGAATAACAGATGTTCATAGACTTATATATAGTATTGATGATGAAAATATCTATGTTGCAAGCTGC
AAAGGACATTATCACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9931.21 Da
• Isoelectric Point: 9.7281

>AT181969 WP_014917131.1 NZ_CP065031:5046199-5046465 [Pectobacterium brasiliense]
MKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTTNNEASV
ASSWKEEW

Nucleotide

• Length: 267 bp

>AT181969 NZ_CP086250:c218752-218498 [Clostridium estertheticum]
ATGATCGCTATAGGTTACTCGAACGCAAGACAAAATTTTAAAAATTATTGTGACAAAGCTACAAAAGATTTTGAAACCAT
TATTATTACTCGAGAGCGTGGTGACAATGTGGTTATGTTATCTGAATCGGCATATAATAACCTTATGGAAAATTTGTACG
TTAGAAGTGATCCCACCTATTATAATGAATTGCTGAAATCTATTGACCAATTAAAAATGGGAAAGGGAATTAGAAAGGAA
CTTCTTGATGAATAA