Detailed information of TA system TA181963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4370893..4371677
• Replicon: chromosome
• Accession: NZ_CP065031
• Organism: Pectobacterium brasiliense strain F126

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: F126LOC_RS19160
• Protein ID: WP_119871144.1
• Coordinates: 4370893..4371387 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: F126LOC_RS19165
• Protein ID: WP_038921611.1
• Coordinates: 4371384..4371677 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F126LOC_RS19135	4366290..4367033	+	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
F126LOC_RS19140	4367092..4367310	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
F126LOC_RS19145	4367313..4367618	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
F126LOC_RS19150	4367818..4368660	-	843	WP_119871146.1	DNA adenine methylase	-
F126LOC_RS19155	4368746..4370467	-	1722	WP_119871145.1	AAA family ATPase	-
F126LOC_RS19160	4370893..4371387	-	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
F126LOC_RS19165	4371384..4371677	-	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
F126LOC_RS19170	4372017..4372259	-	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
F126LOC_RS19175	4372363..4372617	+	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
F126LOC_RS19180	4372702..4372950	+	249	WP_119871143.1	helix-turn-helix domain-containing protein	-
F126LOC_RS19185	4373603..4375357	-	1755	WP_119871142.1	AIPR family protein	-
F126LOC_RS19190	4375585..4376370	-	786	WP_185893700.1	phosphonate metabolism protein PhnP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4363117..4371933	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T181963 WP_119871144.1 NZ_CP065031:c4371387-4370893 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T181963 NZ_CP086220:c5008940-5008662 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT181963 WP_038921611.1 NZ_CP065031:c4371677-4371384 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT181963 NZ_CP086220:c5009203-5008943 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3