Detailed information of TA system TA181962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4367092..4367618
• Replicon: chromosome
• Accession: NZ_CP065031
• Organism: Pectobacterium brasiliense strain F126

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: F126LOC_RS19145
• Protein ID: WP_119871147.1
• Coordinates: 4367313..4367618 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: F126LOC_RS19140
• Protein ID: WP_015855076.1
• Coordinates: 4367092..4367310 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F126LOC_RS19115	4363294..4364556	+	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-
F126LOC_RS19120	4364672..4364866	-	195	WP_044209559.1	DUF3950 domain-containing protein	-
F126LOC_RS19125	4365052..4365393	+	342	WP_119871225.1	hypothetical protein	-
F126LOC_RS19130	4365847..4366197	-	351	WP_158586005.1	hypothetical protein	-
F126LOC_RS19135	4366290..4367033	+	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
F126LOC_RS19140	4367092..4367310	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
F126LOC_RS19145	4367313..4367618	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
F126LOC_RS19150	4367818..4368660	-	843	WP_119871146.1	DNA adenine methylase	-
F126LOC_RS19155	4368746..4370467	-	1722	WP_119871145.1	AAA family ATPase	-
F126LOC_RS19160	4370893..4371387	-	495	WP_119871144.1	GNAT family N-acetyltransferase	-
F126LOC_RS19165	4371384..4371677	-	294	WP_038921611.1	DUF1778 domain-containing protein	-
F126LOC_RS19170	4372017..4372259	-	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
F126LOC_RS19175	4372363..4372617	+	255	WP_119871224.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4363117..4371933	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T181962 WP_119871147.1 NZ_CP065031:4367313-4367618 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T181962 NZ_CP086220:4516768-4517118 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT181962 WP_015855076.1 NZ_CP065031:4367092-4367310 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT181962 NZ_CP086220:4516523-4516774 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46