Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 830973..831613 Replicon chromosome
Accession NZ_CP065031
Organism Pectobacterium brasiliense strain F126

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag F126LOC_RS03670 Protein ID WP_039278477.1
Coordinates 831197..831613 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag F126LOC_RS03665 Protein ID WP_039278475.1
Coordinates 830973..831200 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F126LOC_RS03640 826270..826749 - 480 WP_014916572.1 Hcp family type VI secretion system effector -
F126LOC_RS03645 826874..827371 - 498 WP_039278469.1 M48 family metallopeptidase -
F126LOC_RS03650 827455..828648 - 1194 WP_119872012.1 aspartate/tyrosine/aromatic aminotransferase -
F126LOC_RS03655 828712..829326 - 615 WP_119872013.1 YitT family protein -
F126LOC_RS03660 829577..830056 - 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
F126LOC_RS03665 830973..831200 + 228 WP_039278475.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F126LOC_RS03670 831197..831613 + 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
F126LOC_RS03675 831752..832390 - 639 WP_039517591.1 hypothetical protein -
F126LOC_RS03680 832383..833465 - 1083 WP_119872014.1 alanine racemase -
F126LOC_RS03685 833507..834913 - 1407 WP_010279223.1 replicative DNA helicase -
F126LOC_RS03690 835042..836028 + 987 WP_040033638.1 quinone oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T181956 WP_039278477.1 NZ_CP065031:831197-831613 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T181956 NZ_CP086220:3034836-3035213 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT181956 WP_039278475.1 NZ_CP065031:830973-831200 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT181956 NZ_CP086220:3034415-3034789 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References