Detailed information of TA system TA181954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 159819..160453
• Replicon: chromosome
• Accession: NZ_CP065031
• Organism: Pectobacterium brasiliense strain F126

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: F126LOC_RS00680
• Protein ID: WP_014917023.1
• Coordinates: 159819..160094 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: F126LOC_RS00685
• Protein ID: WP_014917022.1
• Coordinates: 160091..160453 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F126LOC_RS00665	156839..158209	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
F126LOC_RS00670	158206..158904	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
F126LOC_RS00675	159058..159582	+	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
F126LOC_RS00680	159819..160094	+	276	WP_014917023.1	type II toxin-antitoxin system HicA family toxin	Toxin
F126LOC_RS00685	160091..160453	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F126LOC_RS00690	160827..162014	+	1188	WP_080162913.1	sugar transporter	-
F126LOC_RS00695	162087..162989	+	903	WP_119870811.1	CDF family cation-efflux transporter FieF	-
F126LOC_RS00700	163197..164159	+	963	WP_010281567.1	6-phosphofructokinase	-
F126LOC_RS00705	164440..165324	+	885	WP_014917008.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10050.70 Da
• Isoelectric Point: 11.1875

>T181954 WP_014917023.1 NZ_CP065031:159819-160094 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLIQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T181954 NZ_CP086220:c2754000-2753665 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT181954 WP_014917022.1 NZ_CP065031:160091-160453 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT181954 NZ_CP086220:c2754248-2754000 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7