Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 145754..146505 Replicon chromosome
Accession NZ_CP065031
Organism Pectobacterium brasiliense strain F126

Toxin (Protein)


Gene name ataT Uniprot ID A0A433N1V3
Locus tag F126LOC_RS00610 Protein ID WP_010281603.1
Coordinates 146023..146505 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A0M2EWB4
Locus tag F126LOC_RS00605 Protein ID WP_039317395.1
Coordinates 145754..146032 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F126LOC_RS00575 140912..141826 - 915 WP_119870802.1 NAD(P)-dependent oxidoreductase -
F126LOC_RS00580 142164..142922 + 759 WP_119870824.1 DeoR/GlpR family DNA-binding transcription regulator -
F126LOC_RS00585 143143..143541 + 399 WP_127110965.1 hypothetical protein -
F126LOC_RS00590 143538..144386 - 849 WP_119870804.1 DUF4225 domain-containing protein -
F126LOC_RS00595 144388..144867 - 480 WP_039511462.1 Hcp family type VI secretion system effector -
F126LOC_RS00600 145228..145644 + 417 WP_119870805.1 hypothetical protein -
F126LOC_RS00605 145754..146032 + 279 WP_039317395.1 DUF1778 domain-containing protein Antitoxin
F126LOC_RS00610 146023..146505 + 483 WP_010281603.1 GNAT family N-acetyltransferase Toxin
F126LOC_RS00615 146638..147972 + 1335 WP_119870806.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
F126LOC_RS00620 148045..148932 + 888 WP_010281599.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
F126LOC_RS00625 148958..149803 + 846 WP_010281598.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
F126LOC_RS00630 149829..150902 + 1074 WP_119870807.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-144)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17509.30 Da        Isoelectric Point: 9.0439

>T181953 WP_010281603.1 NZ_CP065031:146023-146505 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Download         Length: 483 bp

>T181953 NZ_CP086220:c2459486-2459088 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.12 Da        Isoelectric Point: 4.3611

>AT181953 WP_039317395.1 NZ_CP065031:145754-146032 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITEERQAR
INDLMSRKSPWE

Download         Length: 279 bp

>AT181953 NZ_CP086220:c2459713-2459486 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A433N1V3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2EWB4

References