Detailed information of TA system TA181948

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3002649..3003373
• Replicon: chromosome
• Accession: NZ_CP065030
• Organism: Pectobacterium versatile strain F131

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A855MDQ6
• Locus tag: F131LOC_RS13380
• Protein ID: WP_044205805.1
• Coordinates: 3002649..3002969 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: F131LOC_RS13385
• Protein ID: WP_103972300.1
• Coordinates: 3003038..3003373 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F131LOC_RS13340	2998081..2998959	-	879	WP_103860329.1	cell envelope biogenesis protein OmpA	-
F131LOC_RS13345	2998988..2999524	-	537	WP_103972304.1	hypothetical protein	-
F131LOC_RS13350	2999521..3000063	-	543	Protein_2615	glycoside hydrolase family 108 protein	-
F131LOC_RS13355	3000063..3000176	-	114	WP_103860328.1	phage holin family protein	-
F131LOC_RS13360	3000625..3000834	-	210	WP_133160435.1	hypothetical protein	-
F131LOC_RS13365	3000969..3001373	-	405	WP_103972302.1	tRNA(fMet)-specific endonuclease VapC	-
F131LOC_RS13370	3001373..3001603	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
F131LOC_RS13375	3001697..3002530	-	834	WP_103972301.1	DUF4942 domain-containing protein	-
F131LOC_RS13380	3002649..3002969	-	321	WP_044205805.1	TA system toxin CbtA family protein	Toxin
F131LOC_RS13385	3003038..3003373	-	336	WP_103972300.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F131LOC_RS13390	3003413..3003886	-	474	WP_102118413.1	DNA repair protein RadC	-
F131LOC_RS13395	3004039..3004566	-	528	WP_102118412.1	DUF4234 domain-containing protein	-
F131LOC_RS13400	3004624..3005085	-	462	WP_103972306.1	hypothetical protein	-
F131LOC_RS13405	3005227..3005385	-	159	WP_161569105.1	hypothetical protein	-
F131LOC_RS13410	3005569..3006456	-	888	WP_103972298.1	50S ribosome-binding GTPase	-
F131LOC_RS13415	3006552..3007940	-	1389	WP_103972297.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2996597..3019077	22480
	-	flank	IS/Tn	-	-	2996597..2997706	1109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12092.87 Da
• Isoelectric Point: 4.7768

>T181948 WP_044205805.1 NZ_CP065030:c3002969-3002649 [Pectobacterium versatile]
MQTSPAIPPREDIPCPSPIAVWQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDASVTLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTAVDILRARRATGLLKA

Nucleotide

• Length: 321 bp

>T181948 NZ_CP086220:c1319260-1317938 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12415.10 Da
• Isoelectric Point: 5.1363

>AT181948 WP_103972300.1 NZ_CP065030:c3003373-3003038 [Pectobacterium versatile]
MPSSDTPEWGLKCAVTPRFGARLVQEGNRLHYLADRASFIGMFSKTEARNLERCFSELIKQLEQKLCTGELNPRQQGCVT
LHCDEFTCEADTLGSFGYVYIVIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT181948 NZ_CP086220:c1319526-1319260 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A855MDQ6