Detailed information of TA system TA181947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3000969..3001603
• Replicon: chromosome
• Accession: NZ_CP065030
• Organism: Pectobacterium versatile strain F131

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F131LOC_RS13365
• Protein ID: WP_103972302.1
• Coordinates: 3000969..3001373 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: F131LOC_RS13370
• Protein ID: WP_012765080.1
• Coordinates: 3001373..3001603 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F131LOC_RS13335	2996597..2997706	+	1110	WP_025919286.1	ISAs1 family transposase	-
F131LOC_RS13340	2998081..2998959	-	879	WP_103860329.1	cell envelope biogenesis protein OmpA	-
F131LOC_RS13345	2998988..2999524	-	537	WP_103972304.1	hypothetical protein	-
F131LOC_RS13350	2999521..3000063	-	543	Protein_2615	glycoside hydrolase family 108 protein	-
F131LOC_RS13355	3000063..3000176	-	114	WP_103860328.1	phage holin family protein	-
F131LOC_RS13360	3000625..3000834	-	210	WP_133160435.1	hypothetical protein	-
F131LOC_RS13365	3000969..3001373	-	405	WP_103972302.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F131LOC_RS13370	3001373..3001603	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F131LOC_RS13375	3001697..3002530	-	834	WP_103972301.1	DUF4942 domain-containing protein	-
F131LOC_RS13380	3002649..3002969	-	321	WP_044205805.1	TA system toxin CbtA family protein	-
F131LOC_RS13385	3003038..3003373	-	336	WP_103972300.1	type IV toxin-antitoxin system YeeU family antitoxin	-
F131LOC_RS13390	3003413..3003886	-	474	WP_102118413.1	DNA repair protein RadC	-
F131LOC_RS13395	3004039..3004566	-	528	WP_102118412.1	DUF4234 domain-containing protein	-
F131LOC_RS13400	3004624..3005085	-	462	WP_103972306.1	hypothetical protein	-
F131LOC_RS13405	3005227..3005385	-	159	WP_161569105.1	hypothetical protein	-
F131LOC_RS13410	3005569..3006456	-	888	WP_103972298.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2996597..3019077	22480
	-	flank	IS/Tn	-	-	2996597..2997706	1109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15045.26 Da
• Isoelectric Point: 8.0253

>T181947 WP_103972302.1 NZ_CP065030:c3001373-3000969 [Pectobacterium versatile]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQTYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T181947 NZ_CP086220:1231866-1232042 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT181947 WP_012765080.1 NZ_CP065030:c3001603-3001373 [Pectobacterium versatile]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT181947 NZ_CP086220:1232088-1232504 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4