Detailed information of TA system TA181928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1415305..1415840
• Replicon: plasmid pSymA
• Accession: NZ_CP065021
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I0J99_RS24030
• Protein ID: WP_127665514.1
• Coordinates: 1415565..1415840 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N8JT21
• Locus tag: I0J99_RS24025
• Protein ID: WP_013845222.1
• Coordinates: 1415305..1415565 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS24015	1411493..1411882	+	390	WP_127665516.1	hypothetical protein	-
I0J99_RS24020	1412108..1414861	-	2754	WP_164828692.1	AAA family ATPase	-
I0J99_RS24025	1415305..1415565	+	261	WP_013845222.1	ribbon-helix-helix domain-containing protein	Antitoxin
I0J99_RS24030	1415565..1415840	+	276	WP_127665514.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I0J99_RS24035	1415889..1417043	+	1155	WP_127665513.1	site-specific integrase	-
I0J99_RS24040	1417092..1417391	-	300	WP_013845219.1	hypothetical protein	-
I0J99_RS24045	1417894..1418331	-	438	WP_013845218.1	VOC family protein	-
I0J99_RS24050	1419044..1419349	+	306	Protein_1369	hypothetical protein	-
I0J99_RS24055	1419505..1420452	-	948	WP_013845217.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1514798	1514798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10794.35 Da
• Isoelectric Point: 9.5554

>T181928 WP_127665514.1 NZ_CP065021:1415565-1415840 [Sinorhizobium meliloti]
MKLEWTSKAVADLGRLYDFLAPVNQQAAARTVQSLTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T181928 NZ_CP086213:c5032932-5032177 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9652.01 Da
• Isoelectric Point: 4.8182

>AT181928 WP_013845222.1 NZ_CP065021:1415305-1415565 [Sinorhizobium meliloti]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIDHQAVQAWADSLDSDK
PLSLPL

Nucleotide

• Length: 261 bp

>AT181928 NZ_CP086213:c5033435-5033067 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTGGATGCGATCCGCGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8JT21