Detailed information of TA system TA181927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1325907..1326487
• Replicon: plasmid pSymA
• Accession: NZ_CP065021
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92XP1
• Locus tag: I0J99_RS23635
• Protein ID: WP_010968151.1
• Coordinates: 1326104..1326487 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XCL0
• Locus tag: I0J99_RS23630
• Protein ID: WP_013851145.1
• Coordinates: 1325907..1326107 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS23610	1323116..1323844	-	729	WP_014531163.1	class I SAM-dependent methyltransferase	-
I0J99_RS23615	1323883..1324206	-	324	WP_017266777.1	DUF1127 domain-containing protein	-
I0J99_RS23620	1324302..1325138	+	837	WP_014531162.1	helix-turn-helix transcriptional regulator	-
I0J99_RS23625	1325215..1325616	-	402	WP_014531161.1	GFA family protein	-
I0J99_RS23630	1325907..1326107	+	201	WP_013851145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I0J99_RS23635	1326104..1326487	+	384	WP_010968151.1	type II toxin-antitoxin system VapC family toxin	Toxin
I0J99_RS23640	1327184..1329239	-	2056	Protein_1287	S9 family peptidase	-
I0J99_RS23645	1330236..1331171	+	936	WP_107608029.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1514798	1514798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14292.54 Da
• Isoelectric Point: 6.9172

>T181927 WP_010968151.1 NZ_CP065021:1326104-1326487 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T181927 NZ_CP086213:4070229-4070807 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTTTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7096.22 Da
• Isoelectric Point: 11.1107

>AT181927 WP_013851145.1 NZ_CP065021:1325907-1326107 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT181927 NZ_CP086213:4069586-4070140 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCATCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: Q92XP1

Antitoxin

• AlphaFold DB: A0A7W6MID2