Detailed information of TA system TA181925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1017682..1018289
• Replicon: plasmid pSymA
• Accession: NZ_CP065021
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: I0J99_RS22030
• Protein ID: WP_017264738.1
• Coordinates: 1017682..1017936 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I0J99_RS22035
• Protein ID: WP_040120685.1
• Coordinates: 1017933..1018289 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS22000	1013083..1013787	-	705	WP_014527995.1	haloacid dehalogenase type II	-
I0J99_RS22005	1013878..1014750	+	873	WP_010968043.1	LysR family transcriptional regulator	-
I0J99_RS22010	1014801..1014956	-	156	WP_153320124.1	hypothetical protein	-
I0J99_RS22015	1015042..1015200	-	159	WP_017276730.1	hypothetical protein	-
I0J99_RS22020	1015324..1016364	-	1041	WP_040120687.1	NAD(P)H-binding protein	-
I0J99_RS22025	1016453..1017340	+	888	WP_040120686.1	LysR family transcriptional regulator	-
I0J99_RS22030	1017682..1017936	+	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
I0J99_RS22035	1017933..1018289	+	357	WP_040120685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I0J99_RS22040	1018661..1019317	-	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
I0J99_RS22045	1019347..1020297	-	951	WP_010968047.1	ABC transporter permease	-
I0J99_RS22050	1020300..1021271	-	972	WP_040120683.1	ABC transporter permease	-
I0J99_RS22055	1021288..1022781	-	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1514798	1514798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T181925 WP_017264738.1 NZ_CP065021:1017682-1017936 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T181925 NZ_CP086213:2692431-2693006 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13178.82 Da
• Isoelectric Point: 5.2288

>AT181925 WP_040120685.1 NZ_CP065021:1017933-1018289 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAEARLSA

Nucleotide

• Length: 357 bp

>AT181925 NZ_CP086213:2691964-2692434 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA