Detailed information of TA system TA181924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 874659..875158
• Replicon: plasmid pSymA
• Accession: NZ_CP065021
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: I0J99_RS21345
• Protein ID: WP_010967939.1
• Coordinates: 874659..874832 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I0J99_RS21350
• Protein ID: WP_158605850.1
• Coordinates: 874829..875158 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS21315	870525..871841	+	1317	WP_013845634.1	aspartate aminotransferase family protein	-
I0J99_RS21320	872220..872705	-	486	WP_010967936.1	YidB family protein	-
I0J99_RS21325	872733..872984	-	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
I0J99_RS21330	873579..873938	+	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
I0J99_RS21335	873931..874170	+	240	WP_107608693.1	hypothetical protein	-
I0J99_RS21340	874256..874561	+	306	WP_013845638.1	hypothetical protein	-
I0J99_RS21345	874659..874832	-	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I0J99_RS21350	874829..875158	-	330	WP_158605850.1	damage-inducible protein	Antitoxin
I0J99_RS21355	875093..875278	+	186	WP_013845639.1	hypothetical protein	-
I0J99_RS21360	875519..876889	+	1371	WP_046066760.1	alpha/beta hydrolase	-
I0J99_RS21365	877273..877614	+	342	Protein_832	hypothetical protein	-
I0J99_RS21370	877598..877936	-	339	WP_013845641.1	SDR family oxidoreductase	-
I0J99_RS21375	878053..878496	-	444	WP_010967942.1	PACE efflux transporter	-
I0J99_RS21380	878588..879481	+	894	WP_010967943.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1514798	1514798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• DNA binding HTH domain, AraC-type (196-273)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T181924 WP_010967939.1 NZ_CP065021:c874832-874659 [Sinorhizobium meliloti]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T181924 NZ_CP086213:2069701-2070288 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGAGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12081.74 Da
• Isoelectric Point: 11.1401

>AT181924 WP_158605850.1 NZ_CP065021:c875158-874829 [Sinorhizobium meliloti]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQSAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKVLRALEDT
RPDADDADADAHFQERRAAALRKVAAGDR

Nucleotide

• Length: 330 bp

>AT181924 NZ_CP086213:2070355-2071209 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACATACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAGTTGCTGCATGCGCG
CTACGTCGAGCAGCGTTTCGCCCCCCATGTGCACAGCGGCTTCGCCCTGGTCTTCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGCGGCGGCGAGCACCTGGCGCCCTGGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGACTCCGGCTGGCTCTATCGCGGCTACTACCCGGAGGTGGAGCAGATCGCCAGTATCCTCGACGAGCTGGAAAT
CCGCCACGGCGGGGTGCCGCGTTTCGCCGACAGCGTGGTCCATGACCAGCCCCTGGCGCGCGCCTTCGTCCATCTGCACC
GCTTGCTGGCCGACCACGCCGGCAGCCTGGAAACCCAGACCTGCTGGCGCGAGACGCTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCGGAGGCGCCGAAGGCCGGGTCGGAACCGTTGGCGGTCGCCCGCGCCCGCGAGTTGCTGGCGGCGCGTTT
GCTCGACCCGCCGTCGCTGGAAGAACTGGCGGCTGCGGTGAATCTGTCGCCGTTCCACTTCGCCCGGGTGTTCCGCCGCG
CCACCGGGATGCCGCCGCATGCCTGGCTCCGGCAACGGCGCCTGGAACAGGCCAGGGCGCTGCTCAAGAATGGCCGGGCG
CCGCTGGATGTCGCCCTGGAACTGGGCTTCGCCGACCAGAGCCATCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
GCCGGGCGAGTACCGCGCGGCCTGTGCGCGCTCGTCCTTGACGATGTGCGAATAA

Structures

Toxin

• AlphaFold DB: Q92YA6