Detailed information of TA system TA181923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 502779..503464
• Replicon: plasmid pSymA
• Accession: NZ_CP065021
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I0J99_RS19600
• Protein ID: WP_017263896.1
• Coordinates: 502779..503198 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: I0J99_RS19605
• Protein ID: WP_013845417.1
• Coordinates: 503195..503464 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS19570	498741..499058	+	318	Protein_473	autoinducer-binding protein	-
I0J99_RS19575	499191..499298	-	108	Protein_474	helix-turn-helix domain-containing protein	-
I0J99_RS19580	499586..500958	-	1373	Protein_475	ISNCY family transposase	-
I0J99_RS19585	501228..501608	-	381	WP_003526626.1	transcriptional regulator	-
I0J99_RS19590	501626..501937	-	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
I0J99_RS19595	502212..502625	+	414	WP_014528620.1	hypothetical protein	-
I0J99_RS19600	502779..503198	-	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
I0J99_RS19605	503195..503464	-	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I0J99_RS19610	503602..504072	+	471	Protein_481	LysR family transcriptional regulator	-
I0J99_RS19615	504183..505058	-	876	WP_164828747.1	LysR family transcriptional regulator	-
I0J99_RS19620	506031..506954	-	924	WP_013845420.1	LysR family transcriptional regulator	-
I0J99_RS19625	507071..507940	+	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1514798	1514798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T181923 WP_017263896.1 NZ_CP065021:c503198-502779 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T181923 NZ_CP086213:c160453-160172 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT181923 WP_013845417.1 NZ_CP065021:c503464-503195 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT181923 NZ_CP086213:c160677-160450 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5