Detailed information of TA system TA181922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3572951..3573612
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: I0J99_RS17065
• Protein ID: WP_010970565.1
• Coordinates: 3572951..3573355 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G3Z9
• Locus tag: I0J99_RS17070
• Protein ID: WP_003531793.1
• Coordinates: 3573352..3573612 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS17050	3568487..3569599	+	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-
I0J99_RS17055	3569735..3570442	+	708	WP_107608579.1	phosphatase PAP2 family protein	-
I0J99_RS17060	3570543..3572432	-	1890	WP_014527006.1	MHS family MFS transporter	-
I0J99_RS17065	3572951..3573355	-	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
I0J99_RS17070	3573352..3573612	-	261	WP_003531793.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I0J99_RS17075	3574025..3575059	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
I0J99_RS17080	3575232..3576053	+	822	WP_107608580.1	lytic transglycosylase family protein	-
I0J99_RS17085	3576401..3577084	-	684	WP_010970569.1	carbonic anhydrase	-
I0J99_RS17090	3577258..3578139	-	882	WP_015008003.1	pyridoxal kinase PdxY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T181922 WP_010970565.1 NZ_CP065020:c3573355-3572951 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T181922 NZ_CP086208:200992-201342 [Priestia sp. J2]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9913.20 Da
• Isoelectric Point: 5.1253

>AT181922 WP_003531793.1 NZ_CP065020:c3573612-3573352 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRPE
LDAVFR

Nucleotide

• Length: 261 bp

>AT181922 NZ_CP086208:200706-200987 [Priestia sp. J2]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: H0G3Z9