Detailed information of TA system TA181921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2915340..2915971
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I0J99_RS14120
• Protein ID: WP_013844828.1
• Coordinates: 2915340..2915738 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: I0J99_RS14125
• Protein ID: WP_010970129.1
• Coordinates: 2915738..2915971 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS14085	2910422..2910724	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I0J99_RS14090	2910721..2910996	-	276	WP_107608334.1	type II toxin-antitoxin system ParD family antitoxin	-
I0J99_RS14095	2911282..2911431	+	150	WP_027989811.1	hypothetical protein	-
I0J99_RS14100	2911428..2912363	+	936	WP_107608335.1	site-specific tyrosine recombinase XerD	-
I0J99_RS14105	2912427..2913380	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
I0J99_RS14110	2913625..2915067	+	1443	WP_107608336.1	murein L,D-transpeptidase	-
I0J99_RS14115	2915067..2915306	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
I0J99_RS14120	2915340..2915738	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
I0J99_RS14125	2915738..2915971	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I0J99_RS14130	2916080..2917228	-	1149	WP_107608337.1	GTP-binding protein	-
I0J99_RS14135	2917242..2918324	-	1083	WP_107608338.1	D-alanyl-D-alanine carboxypeptidase	-
I0J99_RS14140	2918576..2919739	+	1164	WP_107608339.1	amidohydrolase	-
I0J99_RS14145	2919762..2920226	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T181921 WP_013844828.1 NZ_CP065020:c2915738-2915340 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T181921 NZ_CP086206:c3431696-3431421 [Pseudomonas sp. HN8-3]
ATGTTGGCCGTTGTTTGGCTTGACGAAGCCATCGGCGACCTGATTGATATCGTGACTTTTATCGCAGCAGAAAACCCCGG
CGCTGCACGACGCATCAAGACCCGCCTGGAAGGCGCGCCTCTACCATTGGCTGAGCACCCCTATCTGTATCCGTGCGGCC
GGGTCCCAGGTACTCGTGAGGTGGTTGTACATCCCAATTATGTGCTGGTGTATAGAGTGACTGCTGAGCGGATCGAAATA
GTCAACGTATTGCACGCCCGGCAAGAATATCCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT181921 WP_010970129.1 NZ_CP065020:c2915971-2915738 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT181921 NZ_CP086206:c3431890-3431696 [Pseudomonas sp. HN8-3]
ATGGGTGTTCCACTTTCCCCAATTGTTTCCGAGTTTGAAACCGAAGAGCAGGCGAGTAGTCATGATCTCTGGTTTCGTAA
AAAAGTCGAAAAAAGCTTGGCCAACCCCGGCCCCGGTGTACCTCATGACGAGGTCATGGCACGAGTTGAGGCGATTATTC
TGAAAGCAGAGCTTGAGCAGCGGGCCAAGGCTTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9