Detailed information of TA system TA181920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2867880..2868646
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: I0J99_RS13855
• Protein ID: WP_003533488.1
• Coordinates: 2868164..2868646 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: I0J99_RS13850
• Protein ID: WP_003533490.1
• Coordinates: 2867880..2868164 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS13835	2863350..2864738	+	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-
I0J99_RS13840	2864742..2866013	+	1272	WP_127541756.1	GNAT family N-acetyltransferase	-
I0J99_RS13845	2866029..2867675	-	1647	WP_107608322.1	AMP-binding protein	-
I0J99_RS13850	2867880..2868164	+	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
I0J99_RS13855	2868164..2868646	+	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
I0J99_RS13865	2869112..2869621	+	510	WP_003533487.1	disulfide bond formation protein B	-
I0J99_RS13870	2869653..2870210	-	558	WP_003533486.1	HNH endonuclease	-
I0J99_RS13875	2870316..2870963	-	648	WP_027993538.1	DNA-3-methyladenine glycosylase	-
I0J99_RS13880	2871116..2872012	+	897	WP_107608323.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
I0J99_RS13885	2872031..2872906	-	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T181920 WP_003533488.1 NZ_CP065020:2868164-2868646 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T181920 NZ_CP086206:c2466978-2466727 [Pseudomonas sp. HN8-3]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAATTGCTCAATGAGCACATGGCATTTACCTGGCCCGAGCTCGTCACGCTGTT
ACGCCGACTCGGATACACACTGATTGAAGGGGCTGGAAGCCGCGTCAAGTTCGATATCGGCGACCCCAGTGCAATGATCA
CGTTGCACAAGCCTCACCCGGGCAACGAACTGAAACATTACATTCGGCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT181920 WP_003533490.1 NZ_CP065020:2867880-2868164 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT181920 NZ_CP086206:c2466730-2466371 [Pseudomonas sp. HN8-3]
GTGAACAACCAGCTCAAACATAAAGGCTACATCGGCTCTATCGAGGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAGAT
TTTATTCATCAAGGCATTAGTGAGCTACGAAGGCAAAACCGTCGCAGAACTGGACGCAGCCTTTCGCGAAGCCGTGGACG
ATTATCTGGCTACCTGCCAGACCCTGGGGCAATCACCTGAGAAGCCATGCAAGGGCTCGTTCAACGTACGCGTTGGGCAT
GAACTGCATCTGGCAGCCGCTCTGGCCGCAATGCGTAAAAAGGTGACGTTGAACGACCTTACGCGCCAAGCGCTGAATGA
GTTTCTGCAACAGCACGACGACGGTCTAGCGGCGGTTTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4