Detailed information of TA system TA181918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2184102..2184756
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: I0J99_RS10450
• Protein ID: WP_003530010.1
• Coordinates: 2184102..2184290 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: I0J99_RS10455
• Protein ID: WP_010969659.1
• Coordinates: 2184295..2184756 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS10435	2180668..2181978	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
I0J99_RS10440	2182003..2182974	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
I0J99_RS10445	2183155..2183925	-	771	WP_010969658.1	hypothetical protein	-
I0J99_RS10450	2184102..2184290	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
I0J99_RS10455	2184295..2184756	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I0J99_RS10460	2185116..2186027	-	912	WP_127545365.1	PhnA-like protein	-
I0J99_RS10465	2186176..2187150	-	975	WP_206379384.1	non-homologous end-joining DNA ligase	-
I0J99_RS10470	2187243..2187413	+	171	WP_206379385.1	hypothetical protein	-
I0J99_RS10475	2187406..2187774	+	369	WP_127545370.1	hypothetical protein	-
I0J99_RS10480	2187918..2188091	-	174	WP_088203163.1	DUF3606 domain-containing protein	-
I0J99_RS10485	2188915..2189118	-	204	WP_026029857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T181918 WP_003530010.1 NZ_CP065020:2184102-2184290 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T181918 NZ_CP086206:c1652478-1652185 [Pseudomonas sp. HN8-3]
ATGACCTATAGCCTTGATTTTGATGCGCGTGCATTGAAGGAATGGCAGAAACTCGGCGACACCCTCCGTCAGCAACTCAA
GAAGAAGCTGATGGAAATCCTGAACAACCCACGTATTGAGGCTAACCGCCTACATGGCCTGCCTGACTGCTACAAGATCA
AACTGCGTAGTAGCCGGTACAGGCTGGTCTATCAAGTGATAGATCAGGAAGTGACCGTGTTCGTGGTCGCTATTGATAAA
CGTGAACGAGATCAGGCTTATCGCAAAGCGTCTGAGCGCCTTGATCAGCGCTAG

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT181918 WP_010969659.1 NZ_CP065020:2184295-2184756 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT181918 NZ_CP086206:c1652707-1652468 [Pseudomonas sp. HN8-3]
TTGGCATCTATCAACATCCGTATCGATGACGACCTGAAAGCTCGCGCCTACCTTGAGCTGGAGAAACTCGGTGTCACCCC
TTCCGAACTGATACGTCAGGCGTTGCAATATGTTGCCGAACGCGGCCAGTTGCCATTTAAGCCTGTCCTCATGACTGAAG
AGGATGAAGCCTTGATGGCGACCGTACGTGAGCGCCTTGCGTCGCCTCAACGCGTGAGGGTTTCTTTGGATGACCTATAG

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0