Detailed information of TA system TA181917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 1371616..1372190
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I0J99_RS06465
• Protein ID: WP_127591098.1
• Coordinates: 1371616..1371981 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92QN3
• Locus tag: I0J99_RS06470
• Protein ID: WP_010969155.1
• Coordinates: 1371975..1372190 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS06440	1366756..1368198	+	1443	WP_010969150.1	NADH-quinone oxidoreductase subunit NuoN	-
I0J99_RS06445	1368195..1368962	+	768	WP_003531861.1	biotin--[acetyl-CoA-carboxylase] ligase	-
I0J99_RS06450	1368990..1370657	+	1668	WP_010969151.1	ribonuclease J	-
I0J99_RS06455	1370716..1371120	+	405	WP_010969152.1	methylmalonyl-CoA epimerase	-
I0J99_RS06460	1371260..1371529	+	270	WP_014526683.1	DUF1467 family protein	-
I0J99_RS06465	1371616..1371981	-	366	WP_127591098.1	type II toxin-antitoxin system VapC family toxin	Toxin
I0J99_RS06470	1371975..1372190	-	216	WP_010969155.1	ribbon-helix-helix protein, CopG family	Antitoxin
I0J99_RS06475	1372258..1372485	-	228	WP_088199345.1	hypothetical protein	-
I0J99_RS06480	1372901..1374229	+	1329	WP_088199346.1	proline--tRNA ligase	-
I0J99_RS06485	1374250..1375560	+	1311	WP_014529722.1	lipoprotein-releasing ABC transporter permease subunit	-
I0J99_RS06490	1375578..1376264	+	687	WP_010969157.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13593.51 Da
• Isoelectric Point: 4.7324

>T181917 WP_127591098.1 NZ_CP065020:c1371981-1371616 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIELPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Nucleotide

• Length: 366 bp

>T181917 NZ_CP086206:c1610037-1609855 [Pseudomonas sp. HN8-3]
GTGGATAGCCGCTATTTGATAGGTAACATCGTTGCAGACGGTTGGTATCTGGTGCGCATCAGGGGCAGTCATCATCACTT
CAAGCACCGGACAAAGCCCGGTCTGGTCACGATTCCTCATCCAAAAAAGGACCTGCTGGATAAAACCGCCAAGAGCATTT
TGAAACAGGCTCTGCTGAGTTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8381.51 Da
• Isoelectric Point: 8.6052

>AT181917 WP_010969155.1 NZ_CP065020:c1372190-1371975 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT181917 NZ_CP086206:c1609832-1609422 [Pseudomonas sp. HN8-3]
ATGCTTTATCCCATCGCAATTTCAACGGGCGACGAGGACCACGCCTGGGGCGTGGAAGTCCCGGATATTCCCGGGTGCTA
TTCCGCCGGCGATGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAGGGGCACTTCGAGATCCTTGCAGAAG
ATGGCGCGCCGATTCCCAGCGCGCAGAAAGTCACCTTGCACGCCGCCAACCCACAGTACGCAGGCTGCACCTGGGCGGTG
GTAGACATCGACGTCACCAAGTACCTGGGCAAGGCGCAAAAACTCAACATCACCCTGCCGGGGTATCTGTTGAATCGCAT
CGATGACTATGTACTGAATCACCCGGAAGAAAAAAGCCGTTCGGGGTTTCTGGCGTCCGCGGCACTCAAGGTATTGCAAC
AGGATCGATAA

Structures

Antitoxin

• AlphaFold DB: Q92QN3