Detailed information of TA system TA181914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 658463..659040
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: I0J99_RS03035
• Protein ID: WP_010968707.1
• Coordinates: 658463..658726 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I0J99_RS03040
• Protein ID: WP_206379443.1
• Coordinates: 658723..659040 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS02995	653792..654232	+	441	WP_010968702.1	DUF2948 family protein	-
I0J99_RS03000	654297..655601	+	1305	WP_010968703.1	histidinol dehydrogenase	-
I0J99_RS03005	655598..656074	+	477	WP_010968704.1	UPF0262 family protein	-
I0J99_RS03010	656088..656567	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
I0J99_RS03015	656725..656943	+	219	WP_004435948.1	translation initiation factor IF-1	-
I0J99_RS03020	657041..657661	+	621	WP_004435951.1	Maf-like protein	-
I0J99_RS03025	657666..657878	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
I0J99_RS03035	658463..658726	+	264	WP_010968707.1	BrnT family toxin	Toxin
I0J99_RS03040	658723..659040	+	318	WP_206379443.1	BrnA antitoxin family protein	Antitoxin
I0J99_RS03045	659243..659707	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
I0J99_RS03050	659704..659892	-	189	WP_013844020.1	hypothetical protein	-
I0J99_RS03055	660212..661312	+	1101	WP_010968710.1	hypothetical protein	-
I0J99_RS03060	661299..662381	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
I0J99_RS03065	662584..662895	+	312	WP_004435964.1	hypothetical protein	-
I0J99_RS03070	663175..663564	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T181914 WP_010968707.1 NZ_CP065020:658463-658726 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T181914 NZ_CP086203:937575-937802 [Morganella morganii]
ATGGCAAGGACGATTGAACGTTCATCTCAGTTTAAAAAAGATTATAAACGGGAAGTTAAGGGTATTTACCGTATGATGTT
AAATGACAGTCTACAGAACATCCTGAATATATTGGTAAATGATCTCCCTATACCTGAAAAATACGCTGATCATCCGCTTA
AAGGAAATTGGCGTACATTTCGTGATTGCCACCTCTATCCTGATTTGATTTTGATTTATAAAAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12242.87 Da
• Isoelectric Point: 10.0883

>AT181914 WP_206379443.1 NZ_CP065020:658723-659040 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLQPEFFKAMEEHRRSRGRPSLEHPKKQLTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT181914 NZ_CP086203:937310-937591 [Morganella morganii]
ATGTCTAAAGTGGAATATGTCAGAGCAAAAATTGATCCGAAGCTGAAAGCAGAAGCGATTGAAACGCTTGAATCGATGGG
CTTAACAGTGAGTGACGCGATAAGAATGTTGATGATTCAGATTGTTGAACAGAAAGCGATGCCATTTTGTGTGAAAAGTA
CACAATATCGTCATGCTGCACGTACCCGGGCCTCAATAGAAGAGTTGGAGTGCGGGAAAGGCAAAAAATTTGACAGTATT
TCAGATTTAATGGCGGATCTGCAGGATGGCAAGGACGATTGA

Structures

Toxin

• AlphaFold DB: Q92S20