Detailed information of TA system TA181913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 492264..492829
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: H0G3P4
• Locus tag: I0J99_RS02325
• Protein ID: WP_003531565.1
• Coordinates: 492584..492829 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: H0G3P5
• Locus tag: I0J99_RS02320
• Protein ID: WP_003531566.1
• Coordinates: 492264..492587 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS02300	488127..488927	+	801	WP_107607982.1	TVP38/TMEM64 family protein	-
I0J99_RS02305	488950..490371	+	1422	WP_013843868.1	NAD(P)/FAD-dependent oxidoreductase	-
I0J99_RS02310	490446..491966	+	1521	WP_027989491.1	HAMP domain-containing histidine kinase	-
I0J99_RS02320	492264..492587	-	324	WP_003531566.1	BrnA antitoxin family protein	Antitoxin
I0J99_RS02325	492584..492829	-	246	WP_003531565.1	BrnT family toxin	Toxin
I0J99_RS02330	493264..493977	+	714	WP_013843872.1	replicative DNA helicase	-
I0J99_RS02335	494162..494857	-	696	WP_027989490.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
I0J99_RS02340	495050..495538	+	489	WP_107607983.1	RidA family protein	-
I0J99_RS02345	495747..496364	+	618	WP_107607984.1	hypothetical protein	-
I0J99_RS02350	496456..497601	+	1146	WP_192937781.1	alpha-hydroxy-acid oxidizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8990.48 Da
• Isoelectric Point: 9.7323

>T181913 WP_003531565.1 NZ_CP065020:c492829-492584 [Sinorhizobium meliloti]
MKVTYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Nucleotide

• Length: 246 bp

>T181913 NZ_CP086203:c893495-893292 [Morganella morganii]
ATGACTAAACTGGACTATCTCATGCGCTTACGTAAGTGCACTTCTATTGAAACACTGGAACGTGTCATTGAGAAGAACAA
ATATGAACTGACTGATGATGAGCTGGAGGTTTTTTATTCAGCAGCCGACCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCATCTGTCTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11933.51 Da
• Isoelectric Point: 10.2830

>AT181913 WP_003531566.1 NZ_CP065020:c492587-492264 [Sinorhizobium meliloti]
MTSLSRRIRHISDKEEAAIQRKIASDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Nucleotide

• Length: 324 bp

>AT181913 NZ_CP086203:c893918-893559 [Morganella morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACCCTCATCAGAAGCAATAATTATTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGGCCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCGTTTAATGACGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAGGGGCATCTCGTCCACTAA

Structures

Toxin

• AlphaFold DB: H0G3P4

Antitoxin

• AlphaFold DB: H0G3P5