Detailed information of TA system TA181909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 46896..47589
• Replicon: plasmid pVIM-1-ZDHY316
• Accession: NZ_CP064944
• Organism: Pseudomonas fulva strain ZDHY316

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: IYR97_RS23145
• Protein ID: WP_000182276.1
• Coordinates: 46896..47252 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: IYR97_RS23150
• Protein ID: WP_001172026.1
• Coordinates: 47254..47589 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IYR97_RS23130	42007..42852	-	846	WP_003464995.1	AraC family transcriptional regulator	-
IYR97_RS23135	43089..46118	-	3030	Protein_50	Tn3 family transposase	-
IYR97_RS23140	46102..46704	-	603	WP_010465829.1	recombinase family protein	-
IYR97_RS23145	46896..47252	+	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IYR97_RS23150	47254..47589	+	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
IYR97_RS23155	47604..47939	+	336	WP_000741275.1	hypothetical protein	-
IYR97_RS23160	47963..48289	+	327	WP_000091614.1	hypothetical protein	-
IYR97_RS23165	48286..48666	+	381	WP_001054412.1	hypothetical protein	-
IYR97_RS23170	48784..50066	+	1283	Protein_57	HD-GYP domain-containing protein	-
IYR97_RS23175	50323..50541	+	219	WP_182275393.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaVIM-1	-	1..50708	50708

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T181909 WP_000182276.1 NZ_CP064944:46896-47252 [Pseudomonas fulva]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T181909 NZ_CP086201:3604351-3604731 [Enterobacter roggenkampii]
ATGCAAACTTTATCGTTATCCGAGCCACGGGCGGCTGAGCCATGCCCGTCGCCCGTGCAGGTCTGGCAGCTGCTGCTCAT
ACATCTGCTAGATAAACATTATGGCCTCACGCTCAACGATACGCCGTTTGGCGATGACAGCGTAATTCAGGCGCACATCA
ACGCCCGTATCTCTCTGTGTGATGCTGTCAATTTCATTGTGCAAAGGTATGAACTGGTGCGCACCGACAACAGCAGTTTT
AACCTTAGCGATCGCTCATCGCTAATTGGTGCCATCGACATTCTGCATGCCCGTCGTGCAACCGGACTGATAGTAAGAAG
CGGCTATAGCAACATAACCCGCATCACAACGGGCCGGTATCAAGGAGGAGAAGCACAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT181909 WP_001172026.1 NZ_CP064944:47254-47589 [Pseudomonas fulva]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT181909 NZ_CP086201:3603994-3604320 [Enterobacter roggenkampii]
ATGCATTCTCAACAATGGGGGCTTACGTGCAGTAAAACGCCCAGCTTCGATGCCAGGCTGGTGCAGGAAGGCAACCGACT
GCACTTTCTGGCTGACCGGGCAGGATTTACCGGCGACTTCAGCGACGATGAAGCACTACGCCTTGACCAAGCTTTTCCGC
TGATAATGAAACAGCTGGAGCAGATGCTTACAACCGGTGAGCTGAATCCTCGCCATCAGCAACGTGTTACCCTGAATCAC
ATTGGACTGACCTGCGAAGCCGACACGCTTGGCTCACACGGTTATGTTTACATCGCGATTTATCCCTCTTCGACTGTAAC
CGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7